Department of Pharmaceutics, ISF College of Pharmacy, Moga, India.
Department of Pharmacology, ISF College of Pharmacy, Moga, India.
J Microencapsul. 2024 Nov;41(7):519-534. doi: 10.1080/02652048.2024.2390951. Epub 2024 Aug 20.
AIM(S): This article explores the application of mesalazine-loaded nanoparticles (MLZ-NPs) encapsulated in plant polysaccharide-based pellets (MLZ-NPs-Pellets) for ulcerative colitis.
MLZ-NPs were prepared and evaluated for diameter, PDI, and entrapment efficiency. efficacy study was conducted on Caco-2 cells. MLZ-NPs were encapsulated in polysaccharides to form MLZ-NPs-Pellets and characterised for efficacy in animals and targeting efficiency in human volunteers.
Optimised batch of MLZ-NPs were characterised for diameter, PDI, zeta potential and entrapment efficiency which was found to be 145.42 ± 6.75 nm, 0.214 ± 0.049, -31.63 mV and 77.65 ± 2.33(%w/w) respectively. ROS, superoxide and NF-kβ were well controlled in Caco-2 cells when treated with MLZ-NPs. data revealed that some parameters (body weight, colon length, lipid peroxidase, and glutathione) recovered significantly in the DSS-induced mice model treated with oral MLZ-NPs-Pellets. Gamma scintigraphy revealed that the formulation can effectively target the colon within 600 min.
MLZ-NPs-Pellets can be effectively used for microbial-triggered colon targeting approach in treating ulcerative colitis.
本文探讨了将载有美沙拉嗪的纳米颗粒（MLZ-NPs）封装在植物多糖基丸剂（MLZ-NPs-Pellets）中应用于溃疡性结肠炎的情况。
制备 MLZ-NPs 并对其粒径、PDI 和包封效率进行评价。在 Caco-2 细胞上进行疗效研究。将 MLZ-NPs 封装在多糖中形成 MLZ-NPs-Pellets，并对其在动物中的疗效和在人类志愿者中的靶向效率进行了表征。
优化批次的 MLZ-NPs 的粒径、PDI、Zeta 电位和包封效率分别为 145.42 ± 6.75nm、0.214 ± 0.049、-31.63mV 和 77.65 ± 2.33(%w/w)。用 MLZ-NPs 处理 Caco-2 细胞时，ROS、超氧化物和 NF-kβ得到了很好的控制。数据显示，用 MLZ-NPs-Pellets 口服治疗 DSS 诱导的小鼠模型后，一些参数（体重、结肠长度、脂质过氧化酶和谷胱甘肽）显著恢复。伽马闪烁成像显示该制剂可在 600 分钟内有效靶向结肠。
MLZ-NPs-Pellets 可有效用于治疗溃疡性结肠炎的微生物触发结肠靶向方法。
