Department of Biology, University of Florida, Gainesville, 32611, Florida, USA.
Botanical Research Institute of Texas, Fort Worth, 76107, Texas, USA.
Am J Bot. 2024 Sep;111(9):e16389. doi: 10.1002/ajb2.16389. Epub 2024 Aug 20.
Reticulate evolution, often accompanied by polyploidy, is prevalent in plants, and particularly in the ferns. Resolving the resulting non-bifurcating histories remains a major challenge for plant phylogenetics. Here, we present a phylogenomic investigation into the complex evolutionary history of the vining ferns, Lygodium (Lygodiaceae, Schizaeales).
Using a targeted enrichment approach with the GoFlag 408 flagellate land plant probe set, we generated large nuclear and plastid sequence datasets for nearly all taxa in the genus and constructed the most comprehensive phylogeny of the family to date using concatenated maximum likelihood and coalescence approaches. We integrated this phylogeny with cytological and spore data to explore karyotype evolution and generate hypotheses about the origins of putative polyploids and hybrids.
Our data and analyses support the origins of several putative allopolyploids (e.g., L. cubense, L. heterodoxum) and hybrids (e.g., L. ×fayae) and also highlight the potential prevalence of autopolyploidy in this clade (e.g., L. articulatum, L. flexuosum, and L. longifolium).
Our robust phylogenetic framework provides valuable insights into dynamic reticulate evolution in this clade and demonstrates the utility of target-capture data for resolving these complex relationships.
在植物中，尤其是在蕨类植物中，普遍存在网状进化，通常伴随着多倍体。解决由此产生的非二分历史仍然是植物系统发育学的主要挑战。在这里，我们对攀援蕨类植物，海金沙（Lygodiaceae，Schizaeales）的复杂进化历史进行了基因组学研究。
使用带有 GoFlag 408 鞭毛陆地植物探针集的靶向富集方法，我们为该属中的几乎所有分类群生成了大型核和质体序列数据集，并使用串联最大似然和聚合法构建了迄今为止该科最全面的系统发育。我们将这个系统发育与细胞学和孢子数据相结合，探索了核型进化，并提出了关于假定的多倍体和杂种起源的假说。
我们的数据和分析支持了几个假定的异源多倍体（例如，L. cubense，L. heterodoxum）和杂种（例如，L. ×fayae）的起源，也突出了这个类群中可能存在的自多倍体（例如，L. articulatum，L. flexuosum 和 L. longifolium）。
我们稳健的系统发育框架为这个类群中的动态网状进化提供了有价值的见解，并展示了目标捕获数据在解决这些复杂关系方面的实用性。
