Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA.
Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, New Jersey, USA.
Antimicrob Agents Chemother. 2024 Oct 8;68(10):e0020824. doi: 10.1128/aac.00208-24. Epub 2024 Aug 20.
We characterized the molecular determinants of meropenem-vaborbactam (MV) non-susceptibility among non-metallo-β-lactamase-producing KPC- (KPC-). Whole-genome sequencing was performed to identify mutations associated with MV non-susceptibility. Isolates with elevated MV MICs were found to have mutations encoding truncated or altered OmpK36 porins and increased copy numbers. KPC- isolates with decreased susceptibility to MV were detected among a collection of isolates predating the availability of MV.
我们对产金属β-内酰胺酶的（KPC-）肺炎克雷伯菌中不敏感的美罗培南-沃诺拉赞（MV）的分子决定因素进行了特征描述。进行全基因组测序以鉴定与 MV 不敏感相关的突变。发现具有升高的 MV MIC 的分离株具有编码截短或改变的 OmpK36 孔蛋白的突变和增加的 拷贝数。在 MV 可用之前收集的分离株中检测到对 MV 敏感性降低的 KPC- 分离株。
