对产 KPC 耐美罗培南-沃诺拉赞的分子分析。
A molecular analysis of meropenem-vaborbactam non-susceptible KPC-producing .
机构信息
Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA.
Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, New Jersey, USA.
出版信息
Antimicrob Agents Chemother. 2024 Oct 8;68(10):e0020824. doi: 10.1128/aac.00208-24. Epub 2024 Aug 20.
Abstract
We characterized the molecular determinants of meropenem-vaborbactam (MV) non-susceptibility among non-metallo-β-lactamase-producing KPC- (KPC-). Whole-genome sequencing was performed to identify mutations associated with MV non-susceptibility. Isolates with elevated MV MICs were found to have mutations encoding truncated or altered OmpK36 porins and increased copy numbers. KPC- isolates with decreased susceptibility to MV were detected among a collection of isolates predating the availability of MV.
摘要
我们对产金属β-内酰胺酶的（KPC-）肺炎克雷伯菌中不敏感的美罗培南-沃诺拉赞（MV）的分子决定因素进行了特征描述。进行全基因组测序以鉴定与 MV 不敏感相关的突变。发现具有升高的 MV MIC 的分离株具有编码截短或改变的 OmpK36 孔蛋白的突变和增加的 拷贝数。在 MV 可用之前收集的分离株中检测到对 MV 敏感性降低的 KPC- 分离株。
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本文引用的文献
1
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Pharmaceuticals (Basel). 2022 Apr 12;15(4):463. doi: 10.3390/ph15040463.
2
Treatment for carbapenem-resistant Enterobacterales infections: recent advances and future directions.碳青霉烯类耐药肠杆菌科感染的治疗：最新进展和未来方向。
Eur J Clin Microbiol Infect Dis. 2021 Oct;40(10):2053-2068. doi: 10.1007/s10096-021-04296-1. Epub 2021 Jun 24.
3
New Carbapenemase Inhibitors: Clearing the Way for the β-Lactams.新型碳青霉烯酶抑制剂：为β-内酰胺类药物开辟道路。
Int J Mol Sci. 2020 Dec 6;21(23):9308. doi: 10.3390/ijms21239308.
4
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Infect Dis Clin North Am. 2020 Dec;34(4):773-819. doi: 10.1016/j.idc.2020.05.001. Epub 2020 Sep 30.
5
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