State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Xianyang, Shaanxi, China.
Xinjiang Production and Construction Crops Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, College of Life Sciences, Tarim University, Xinjiang, China.
Microbiol Spectr. 2024 Oct 3;12(10):e0071224. doi: 10.1128/spectrum.00712-24. Epub 2024 Aug 20.
, a soil-borne fungus, is the cause of the devastating wheat disease known as wheat crown rot (WCR). The persistence of this pathogen in the soil and crop residues contributes to the increased occurrence and severity of WCR. Therefore, developing effective strategies to prevent and manage WCR is of great importance. In this study, we isolated a bacterial strain, designated as SR9, from the stem of wheat, that exhibited potent antagonistic effects against , as well as the biocontrol efficacy of SR9 on WCR was quantified at 83.99% ± 0.11%. We identified SR9 as and demonstrated its potential as a plant probiotic. SR9 displayed broad-spectrum antagonism against other fungal pathogens, including , , and . Whole-genome sequencing analysis revealed that SR9 harbored genes encoding various cell wall-degrading enzymes, cellulases, and lipases, along with antifungal metabolites, which are responsible for its antagonistic activity. Gene knockout and quantitative PCR analyses reveal that phenazine is the essential factor for antagonism. SR9 possessed genes related to auxin synthesis, flagellar biosynthesis, biofilm adhesion, and the chemotaxis system, which play pivotal roles in plant colonization and growth promotion; we also evaluated the effects of SR9 on plant growth in wheat and . Our findings strongly suggest that SR9 holds great promise as a biocontrol agent for WCR in sustainable agriculture.IMPORTANCEThe escalating prevalence of wheat crown rot, primarily attributed to , necessitates the development of cost-effective and eco-friendly biocontrol strategies. While plant endophytes are recognized for their biocontrol potential, reports on effective strains targeting wheat crown rot are sparse. This study introduces the SR9 strain as an efficacious antagonist to the wheat crown rot pathogen . Demonstrating a significant reduction in wheat crown rot incidence and notable plant growth promotion, SR9 emerges as a key contributor to plant health and agricultural sustainability. Our study outlines a biological approach to tackle wheat crown rot, establishing a groundwork for innovative sustainable agricultural practices.
, 一种土传真菌，是导致毁灭性小麦病害——小麦冠腐病（WCR）的原因。这种病原体在土壤和作物残体中的持续存在导致了 WCR 的发生率和严重程度的增加。因此，开发有效的预防和管理 WCR 的策略非常重要。在这项研究中，我们从小麦茎部分离出一株细菌菌株，命名为 SR9，它对 表现出强烈的拮抗作用，SR9 对 WCR 的防治效果为 83.99%±0.11%。我们鉴定出 SR9 为 ，并证明了它作为植物益生菌的潜力。SR9 对其他真菌病原体如 、 、 表现出广谱拮抗作用。全基因组测序分析表明，SR9 携带编码各种细胞壁降解酶、纤维素酶和脂肪酶以及抗真菌代谢物的基因，这些基因是其拮抗活性的原因。基因敲除和定量 PCR 分析表明，吩嗪是拮抗作用的必需因素。SR9 拥有与生长素合成、鞭毛生物合成、生物膜粘附和趋化系统相关的基因，这些基因在植物定殖和生长促进中发挥着关键作用；我们还评估了 SR9 对小麦和 的植物生长的影响。我们的研究结果强烈表明，SR9 作为可持续农业中 WCR 的生物防治剂具有巨大的潜力。
重要性：小麦冠腐病的发病率不断上升，主要归因于 ，因此需要开发经济高效和环保的生物防治策略。虽然植物内生菌因其生物防治潜力而受到认可，但针对小麦冠腐病的有效菌株的报道很少。本研究介绍了 SR9 菌株作为小麦冠腐病病原体的有效拮抗剂。该菌株显著降低了小麦冠腐病的发病率，并显著促进了植物生长，是植物健康和农业可持续性的关键因素。我们的研究概述了一种应对小麦冠腐病的生物方法，为创新的可持续农业实践奠定了基础。
