da Silva Fábio Gonçalves, Moura Drielly Rodrigues, de Oliveira Pâmela Maria, de Souza Cardoso Quintão Tatyane, Cassemiro Évelin Mota, de Campos Gabriel Montenegro, da Silva Anielly Sarana, Bezerra Rafael Dos Santos, de Noronha Lorena Aparecida Gonçalves, Ramalho Walter Massa, de Araújo Wildo Navegantes, Pereira Alex Leite, Slavov Svetoslav Nanev, Haddad Rodrigo
Center for Tropical Medicine, University of Brasília, Federal District, Brasília, Brazil.
Molecular Diagnostics Laboratory, University Hospital of Brasília/EBSERH, Federal District, Brasília, Brazil.
Braz J Microbiol. 2024 Dec;55(4):3279-3286. doi: 10.1007/s42770-024-01490-z. Epub 2024 Aug 21.
Human Pegivirus Type 1 (HPgV-1), a ubiquitous commensal virus, has been recently suggested as a marker of immunologic function. There is scarce data for the presence, genotypes, and molecular characteristics of HPgV-1 among kidney transplant recipients. Therefore, the objective of this study was to examine the prevalence and the molecular characteristics (cycle threshold, genotypes) of this viral infection among kidney transplant recipients from the Brasília, Federal District of Brazil. HPgV-1 RNA detection in the plasma was assessed by RT-qPCR. Positive samples were submitted to sequencing and phylogenetic analysis of the 5´-UTR portion of the viral genome. The estimated HPgV-1 prevalence among renal-transplant recipients was 20%. The performed phylogenetic inference revealed that the most frequent genotype among these patients was HPgV-1 genotype 2 (78.9%) presented by its two subgenotypes (2 A and 2B), followed by genotypes 1 and 3 (10.5% each). This study presents new data about the HPgV-1 circulation and molecular characteristics among kidney transplant recipients from the Federal District of Brazil. Further work is fundamental to examine the effect of HPgV-1 among patients with immunological suppression, including kidney transplant recipients.
人1型佩吉病毒（HPgV-1）是一种普遍存在的共生病毒，最近被认为是免疫功能的一个标志物。关于肾移植受者中HPgV-1的存在情况、基因型和分子特征的数据很少。因此，本研究的目的是调查巴西联邦区巴西利亚肾移植受者中这种病毒感染的患病率和分子特征（循环阈值、基因型）。通过逆转录定量聚合酶链反应（RT-qPCR）评估血浆中HPgV-1 RNA的检测情况。对阳性样本进行病毒基因组5′-非翻译区（5´-UTR）部分的测序和系统发育分析。肾移植受者中HPgV-1的估计患病率为20%。进行的系统发育推断显示，这些患者中最常见的基因型是HPgV-1 2型（78.9%），由其两个亚型（2 A和2B）呈现，其次是1型和3型（各占10.5%）。本研究提供了关于巴西联邦区肾移植受者中HPgV-1传播和分子特征的新数据。进一步的研究对于研究HPgV-1对包括肾移植受者在内的免疫抑制患者的影响至关重要。
