巴西暴发性肝炎患者中人类pegivirus-1的检测与基因分型
Human Pegivirus-1 Detection and Genotyping in Brazilian Patients with Fulminant Hepatitis.
作者信息
da Silva Anielly Sarana, de Campos Gabriel Montenegro, Villanova Marcia Guimarães, Bezerra Rafael Dos Santos, Santiago Luciana Maria Mendes, Haddad Rodrigo, Covas Dimas Tadeu, Giovanetti Marta, Alcantara Luiz Carlos Junior, Elias Maria Carolina, Sampaio Sandra Coccuzzo, Kashima Simone, Slavov Svetoslav Nanev
机构信息
Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14051-140, SP, Brazil.
Department of Gastroenterology, University Hospital, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14048-900, SP, Brazil.
出版信息
Pathogens. 2023 Sep 1;12(9):1122. doi: 10.3390/pathogens12091122.
Fulminant hepatitis is a severe clinical disease characterized by a marked decline in liver function and encephalopathy. In a previous survey, using metagenomics in a group of 27 patients with this clinical condition, we observed an expressive quantity of reads of the Human pegivirus-1 (HPgV-1). Therefore, the objective of this study was to evaluate the frequency, molecular features, and HPgV-1 circulating genotypes in patients with fulminant hepatitis. After testing the collected plasma samples, we discovered twelve samples (44.4%) that were positive for HPgV-1 RNA (using both real-time and nested PCR). The positive samples presented a mean cycle threshold (Ct) of 28.5 (±7.3). Genotyping assignments revealed that all HPgV-1 positive samples belonged to the HPgV-1 genotype 2 (both subgenotypes 2A and 2B were identified). Although HPgV-1 is considered a commensal virus, little is known regarding its prevalence and genotypes in cases of fulminant hepatitis. More research is needed to understand whether HPgV-1 can be implicated in clinical disorders and infectious diseases.
暴发性肝炎是一种严重的临床疾病，其特征为肝功能显著下降和肝性脑病。在之前的一项调查中，我们对一组27例患有这种临床病症的患者使用宏基因组学方法，观察到人类pegivirus - 1（HPgV - 1）的大量读数。因此，本研究的目的是评估暴发性肝炎患者中HPgV - 1的频率、分子特征和循环基因型。在对收集的血浆样本进行检测后，我们发现12个样本（44.4%）HPgV - 1 RNA呈阳性（采用实时PCR和巢式PCR两种方法）。阳性样本的平均循环阈值（Ct）为28.5（±7.3）。基因分型结果显示，所有HPgV - 1阳性样本均属于HPgV - 1基因型2（同时鉴定出2A和2B两个亚型）。尽管HPgV - 1被认为是一种共生病毒，但关于其在暴发性肝炎病例中的流行情况和基因型，人们了解甚少。需要更多的研究来了解HPgV - 1是否与临床疾病和传染病有关。
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