Helminthology Laboratory & Molecular Taxonomy Research Unit, Department of Zoology, University of Burdwan, Golapbag, Burdwan, 713104, India.
Department of Zoology, University of Burdwan, Golapbag, Burdwan, 713104, India.
Acta Parasitol. 2024 Sep;69(3):1492-1500. doi: 10.1007/s11686-024-00879-y. Epub 2024 Aug 20.
The influence of two key factors, host length and infection site, on the host-parasite interaction in Ompok bimaculatus (Butter catfish) from Mukutmanipur Dam Lake, were investigated.
Present study involved 192 specimens of Ompok bimaculatus with varying body lengths, subjected to diverse statistical analyses. One-way analysis of variance (ANOVA) was performed for the parasite numbers for three groups (cestode, nematode and trematode). Subsequently, we conducted one-way permutational multivariate analysis of variance (PERMANOVA) followed by pairwise test to assess parasite numbers across three body sites (intestine, mesentery, and bodycavity), employing the Bray-Curtis index. Additionally, Principal Coordinate Analysis (PCoA) for the same dataset was performed using the same index. Linear regression analysis was performed for the fish length-cestode number, fish length-nematode number, fish length-trematode number and fish length-total parasite number.
One-way ANOVA revealed no significant differences in parasite numbers among the three endo-helminth groups (cestode, nematode, and trematode). The results of PERMANOVA revealed significant differences in parasite numbers across the three body sites of the host fishes (groups) (F = 9.41, p = 0.0001). Pairwise tests further demonstrated significant differences between the intestine-mesentery, intestine-body-cavity, and mesentery-body-cavity. Additionally, Principal Coordinate Analysis (PCoA) unveiled a significant relationship between infection site and parasite number. However, linear regression analysis examining the relationship between fish length and parasite abundance indicated no significant associations.
Through a detailed exploration of the statistical analyses, we provide insights into the host-parasite interaction, elucidating both established knowledge and novel findings in fish parasitology.
本研究旨在探讨宿主长度和感染部位这两个关键因素对 Mukutmanipur 大坝湖鲷（Butter catfish）体内寄生虫相互作用的影响。
本研究共涉及 192 条不同体长的鲷鱼样本，进行了多种统计分析。采用单因素方差分析（ANOVA）对三组寄生虫（绦虫、线虫和吸虫）的数量进行分析。随后，我们进行了单向可置换多元方差分析（PERMANOVA），并结合两两检验，采用 Bray-Curtis 指数评估三个体部（肠道、肠系膜和体腔）的寄生虫数量。此外，我们还对同一数据集进行了基于相同指数的主坐标分析（PCoA）。同时，还对鱼类体长-绦虫数量、鱼类体长-线虫数量、鱼类体长-吸虫数量和鱼类体长-总寄生虫数量进行了线性回归分析。
单因素 ANOVA 结果显示，三组内寄生虫（绦虫、线虫和吸虫）的数量之间没有显著差异。PERMANOVA 结果显示，宿主鱼类（组）三个体部的寄生虫数量存在显著差异（F = 9.41，p = 0.0001）。两两检验进一步表明，肠道-肠系膜、肠道-体腔和肠系膜-体腔之间存在显著差异。此外，主坐标分析（PCoA）揭示了感染部位与寄生虫数量之间存在显著关系。然而，线性回归分析表明，鱼类体长与寄生虫丰度之间没有显著关联。
通过对统计分析的详细研究，我们深入了解了宿主-寄生虫相互作用，为鱼类寄生虫学提供了既有知识和新发现。
