在布基纳法索中部地区的一个村庄进行的杀虫剂抗性纵向调查显示，在斑蚊属和按蚊属中同时存在 1014F、1014S 和 402L 突变。

Longitudinal survey of insecticide resistance in a village of central region of Burkina Faso reveals co-occurrence of 1014F, 1014S and 402L mutations in Anopheles coluzzii and Anopheles arabiensis.

机构信息

Department of Public Health and Infectious Diseases, Sapienza University of Rome, Rome, Italy.

Centre National de Recherche et Formation Sur le Paludisme, Ouagadougou, Burkina Faso.

出版信息

Malar J. 2024 Aug 20;23(1):250. doi: 10.1186/s12936-024-05069-9.

DOI:10.1186/s12936-024-05069-9

PMID:39164725

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11334353/

Abstract

BACKGROUND

Pyrethroid resistance is one of the major threats for effectiveness of insecticide-treated bed nets (ITNs) in malaria vector control. Genotyping of mutations in the voltage gated sodium channel (VGSC) gene is widely used to easily assess the evolution and spread of pyrethroid target-site resistance among malaria vectors. L1014F and L1014S substitutions are the most common and best characterized VGSC mutations in major African malaria vector species of the Anopheles gambiae complex. Recently, an additional substitution involved in pyrethroid resistance, i.e. V402L, has been detected in Anopheles coluzzii from West Africa lacking any other resistance alleles at locus 1014. The evolution of target-site resistance mutations L1014F/S and V402L was monitored in An. coluzzii and Anopheles arabiensis specimens from a Burkina Faso village over a 10-year range after the massive ITN scale-up started in 2010.

METHODS

Anopheles coluzzii (N = 300) and An. arabiensis (N = 362) specimens collected both indoors and outdoors by different methods (pyrethrum spray catch, sticky resting box and human landing collections) in 2011, 2015 and 2020 at Goden village were genotyped by TaqMan assays and sequencing for the three target site resistance mutations; allele frequencies were statistically investigated over the years.

RESULTS

A divergent trend in resistant allele frequencies was observed in the two species: 1014F decreased in An. coluzzii (from 0.76 to 0.52) but increased in An. arabiensis (from 0.18 to 0.70); 1014S occurred only in An. arabiensis and slightly decreased over time (from 0.33 to 0.23); 402L increased in An. coluzzii (from 0.15 to 0.48) and was found for the first time in one An. arabiensis specimen. In 2020 the co-occurrence of different resistance alleles reached 43% in An. coluzzii (alleles 410L and 1014F) and 32% in An. arabiensis (alleles 1014F and 1014S).

CONCLUSIONS

Overall, an increasing level of target-site resistance was observed among the populations with only 1% of the two malaria vector species being wild type at both loci, 1014 and 402, in 2020. This, together with the co-occurrence of different mutations in the same specimens, calls for future investigations on the possible synergism between resistance alleles and their phenotype to implement local tailored intervention strategies.

摘要

背景

拟除虫菊酯类杀虫剂抗性是影响杀虫剂处理过的蚊帐（ITNs）控制疟疾媒介效能的主要威胁之一。电压门控钠离子通道（VGSC）基因突变的基因分型广泛用于评估疟疾媒介中拟除虫菊酯类杀虫剂靶标抗性的进化和传播。L1014F 和 L1014S 取代是冈比亚按蚊复合体中主要非洲疟疾媒介物种中最常见和特征最好的 VGSC 突变。最近，在西非没有其他 1014 位抗性等位基因的按蚊库蚊中，检测到了一个与拟除虫菊酯类杀虫剂抗性相关的额外替代物，即 V402L。在 2010 年大规模推广 ITN 之后的 10 年内，监测了在布基纳法索一个村庄的按蚊库蚊和按蚊 arabiensis 标本中 L1014F/S 和 V402L 靶标抗性突变的进化。

方法

2011 年、2015 年和 2020 年，在戈登村采用不同方法（除虫菊酯喷雾捕捉、粘性休息盒和人类降落收集）室内外共收集 300 只按蚊库蚊和 362 只按蚊 arabiensis 标本，通过 TaqMan 检测和测序对三种靶标抗性突变进行基因分型；多年来统计分析等位基因频率。

结果

两种物种的抗性等位基因频率呈现出不同的趋势：1014F 在按蚊库蚊中减少（从 0.76 降至 0.52），但在按蚊 arabiensis 中增加（从 0.18 增至 0.70）；1014S 仅在按蚊 arabiensis 中发生，且随时间略有下降（从 0.33 降至 0.23）；402L 在按蚊库蚊中增加（从 0.15 增至 0.48），且首次在一只按蚊 arabiensis 标本中发现。2020 年，按蚊库蚊中不同抗性等位基因的共现率达到 43%（等位基因 410L 和 1014F），按蚊 arabiensis 为 32%（等位基因 1014F 和 1014S）。