Department of Dermatology and Allergology, University Hospital Augsburg, Augsburg, Germany.
Institute of Mathematics, University of Augsburg, Augsburg, Germany.
Int Wound J. 2024 Aug;21(8):e70015. doi: 10.1111/iwj.70015.
The aim of this study was to investigate acute wound healing with dynamic optical coherence tomography (D-OCT). From 22 patients with 23 split skin graft donor sites, vessels at four wound edges, the wound bed, and adjacent and unaffected skin of the contralateral leg were measured by D-OCT at six time points from surgery to 4 weeks of healing. Changes in vessel orientation, density, diameter, morphology and pattern in horizontal, vertical and 3D images were analysed for wound healing and re-epithelialization. At 300 μm depth, there were significant differences of blobs and serpiginous vessels between normal and wounded skin. The wound had significantly more vertically oriented vessels, a higher degree of branching, vessel density and diameter compared with healthy skin. 3D images showed increased angiogenesis from healthy skin towards the wound centre, significantly higher vessel density at the wound than at normal skin and the highest at the interface. During wound healing blobs, coils and serpiginous vessels occurred significantly more frequently in lesional than healthy skin. Vessel density was greatest at the beginning, decreased and then increased by 4 weeks post-surgery. D-OCT helps to evaluate acute wound healing by visualizing and quantifying blood vessel growth in addition to re-epithelialization.
本研究旨在通过动态光学相干断层扫描（D-OCT）来研究急性伤口愈合。从 22 名接受 23 处皮肤切开供体部位的患者中，在手术至愈合 4 周的 6 个时间点，通过 D-OCT 测量了四个伤口边缘、伤口床以及对侧腿部相邻和未受影响的皮肤的血管。分析了水平、垂直和 3D 图像中血管取向、密度、直径、形态和模式的变化，以研究伤口愈合和再上皮化。在 300μm 深度，正常皮肤和受伤皮肤之间存在明显的斑点和蜿蜒血管差异。与健康皮肤相比，伤口具有明显更多的垂直取向血管、更高的分支程度、血管密度和直径。3D 图像显示，从健康皮肤向伤口中心有增加的血管生成，在伤口处的血管密度明显高于正常皮肤，在界面处最高。在伤口愈合过程中，斑点、线圈和蜿蜒血管在病变皮肤中比在健康皮肤中更频繁地出现。在手术后 4 周，血管密度最初最大，然后减少，最后增加。D-OCT 通过可视化和定量血管生成，除了再上皮化之外，有助于评估急性伤口愈合。
