Evaluation of Ethanolic Extract of and Trehalose on Cryopreserved Goat Epididymal Spermatozoa.

The purpose of this study, carried out in two experiments, was to investigate the antioxidant effect of in Experiment 1, and trehalose in Experiment 2 in the freezing extender, on the quality of frozen-thawed goat epididymal spermatozoa. Sperm samples were added to based egg-yolk Tris-extender containing experimental treatments. The first experimental treatments included the following: an extender of the control group without additive and extender containing 50, 100, or 150 µg/mL of ethanolic extract (PEE1, PEE2, and PEE3, respectively). Treatments of the second experiment include an extender of the control group without additive, an extender containing 100 mM of trehalose (Tr), an extender containing 100 µg/mL PEE2, and 100 µg/mL PEE2 + 100 mM Tr. The results of the first experiment showed that PEE2 compared with the control group led to a significant decrease ( < 0.05) in malondialdehyde (MDA) concentration. Also, PEE1 and PEE2 treatments resulted in a significant increase ( < 0.05) in motility parameters by computer-assisted sperm analysis, and MDA concentrations decreased significantly ( < 0.05) in all treatments compared with the control group. In general, the results of the present experiment showed that ethanolic extract at the level of 100 µg/mL was effective in improving the quality of frozen-thawed goat epididymal spermatozoa. Also, a combination of ethanolic extract and trehalose can be successful in freezing goat epididymal spermatozoa.