Heidarzadeh Reza, Tabatabaei Vakili Saleh
Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
Vet Med Sci. 2025 May;11(3):e70370. doi: 10.1002/vms3.70370.
Oxidative stress, caused by an imbalance between reactive oxygen species (ROS) and antioxidants, poses a significant threat to sperm quality during storage.
This study aimed to evaluate the protective effects of cinnamon extract, a natural antioxidant, on ram sperm quality during liquid storage at 4°C.
Semen samples from 10 rams were collected, diluted and divided into five treatment groups. Each group received different concentrations of cinnamon extract (0, 50, 100, 150 and 200 µL/mL) added to the diluted semen. Sperm quality was evaluated at storage intervals of 1, 24, 48 and 72 h, with all samples maintained at 4°C during the storage period.
This study demonstrated that cinnamon extract significantly enhances ram sperm quality during liquid storage at 4°C. Notably, the addition of cinnamon extract, particularly at a concentration of 100 µL/mL, resulted in substantial improvements in progressive motility, total motility, sperm viability and plasma membrane integrity over the storage period. Specifically, after 1 h, the 50 µL/mL group showed a significant increase in progressive motility. At 24 h, both total and progressive motility were significantly higher in the 50, 100 and 150 µL/mL groups. By 48 h, the 50 and 100 µL/mL groups demonstrated significant enhancements in all measured parameters. The optimal results were observed at 72 h, where the 100 µL/mL group exhibited the highest values across all sperm quality metrics. These findings underscore the effectiveness of cinnamon extract as a natural antioxidant for preserving sperm quality during storage.
Supplementation with cinnamon extract significantly enhances ram sperm quality, with an optimal concentration of 100 µL/mL effectively maintaining sperm viability during 72 h of liquid storage. These results suggest that cinnamon extract may serve as a valuable natural antioxidant for improving sperm preservation in liquid storage conditions.
活性氧（ROS）与抗氧化剂之间的失衡所导致的氧化应激，在精液储存期间对精子质量构成重大威胁。
本研究旨在评估天然抗氧化剂肉桂提取物对4℃液体储存期间公羊精子质量的保护作用。
采集10只公羊的精液样本，进行稀释并分为五个处理组。每组在稀释后的精液中添加不同浓度的肉桂提取物（0、50、100、150和200μL/mL）。在储存1、24、48和72小时的时间间隔评估精子质量，所有样本在储存期间保持在4℃。
本研究表明，肉桂提取物在4℃液体储存期间显著提高公羊精子质量。值得注意的是，添加肉桂提取物，特别是浓度为100μL/mL时，在储存期间精子的前向运动率、总运动率、精子活力和质膜完整性有显著改善。具体而言，1小时后，50μL/mL组的前向运动率显著增加。24小时时，50、100和150μL/mL组的总运动率和前向运动率均显著更高。到48小时时，50和100μL/mL组在所有测量参数上均表现出显著增强。在72小时观察到最佳结果，其中100μL/mL组在所有精子质量指标上均表现出最高值。这些发现强调了肉桂提取物作为一种天然抗氧化剂在储存期间保存精子质量方面的有效性。
补充肉桂提取物显著提高公羊精子质量，最佳浓度为100μL/mL，可在72小时液体储存期间有效维持精子活力。这些结果表明，肉桂提取物可能是一种有价值的天然抗氧化剂，可改善液体储存条件下的精子保存。
Zotero 插件
