School of Biotechnology and Food Engineering, Changshu Institute of Technology, Suzhou 215500, China.
Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China.
J Agric Food Chem. 2024 Sep 4;72(35):19526-19536. doi: 10.1021/acs.jafc.4c04487. Epub 2024 Aug 21.
Phloretin is a natural dihydrochalcone (DHC) that exhibits various pharmacological and therapeutic activities. Rehd. () is widely planted in the middle of China and its leaves contain an extremely high content of phloridzin, a glycosylated derivative of phloretin. In the present study, we observed a significant increase in phloretin content in the leaves of planted at high altitudes. To investigate the mechanisms of phloretin accumulation, we explored changes in the proteome profiles of plants grown at various altitudes. The results showed that at high altitudes, photosynthesis- and DHC biosynthesis-related proteins were downregulated and upregulated, respectively, leading to reduced chlorophyll content and DHC accumulation in the leaves. Moreover, we identified a novel phloridzin-catalyzing glucosidase whose expression level was significantly increased in high-altitude-cultivated plants. This work provided a better understanding of the mechanism of phloretin accumulation and effective and economic strategies for phloretin production.
根皮苷是一种天然二氢查耳酮（DHC），具有多种药理和治疗活性。（）广泛种植于中国中部，其叶子含有极高含量的根皮苷，这是根皮素的糖基化衍生物。在本研究中，我们观察到高海拔种植的叶子中根皮素含量显著增加。为了研究根皮素积累的机制，我们探索了不同海拔高度生长的植物的蛋白质组谱变化。结果表明，在高海拔地区，与光合作用和 DHC 生物合成相关的蛋白质分别下调和上调，导致叶片中叶绿素含量和 DHC 积累减少。此外，我们鉴定出一种新型的根皮苷催化葡萄糖苷酶，其在高海拔种植植物中的表达水平显著增加。这项工作更好地理解了根皮素积累的机制，并为根皮素的生产提供了有效和经济的策略。
