University of Tennessee College of Veterinary Medicine, Knoxville, TN, USA.
J Vet Diagn Invest. 2024 Nov;36(6):910-914. doi: 10.1177/10406387241271362. Epub 2024 Aug 21.
A geriatric captive bobcat () was euthanized due to progressive anorexia and lethargy. Meningoencephalitis with intralesional apicomplexan organisms was identified histologically. With immunohistochemistry, the organisms were immunolabeled by anti- antibodies. PCR targeting the ITS region of the parasite yielded an amplicon with >99.6% identity to several , , and sequences. Amplification of the 18S region yielded a sequence that was 99.9% similar to sequences of both (MN169125) and (KX470746). Inflammatory disease of the CNS due to sp. infection is uncommonly reported in felids and has not been reported previously in bobcats, to our knowledge. Here, we briefly review associated CNS disease in other felids, confirm that it can affect bobcats, and highlight the challenges of species-level identification of sp. in routine diagnostic work.
一只老年圈养的短尾猫（）因进行性厌食和嗜睡而被安乐死。组织病理学鉴定为脑膜脑炎伴腔内顶复门虫。免疫组织化学染色显示，这些生物体被抗抗体免疫标记。针对寄生虫 ITS 区的 PCR 产生了一个与多个、和序列具有 >99.6%同一性的扩增子。扩增 18S 区得到的序列与（MN169125）和（KX470746）的序列均具有 99.9%的相似性。据我们所知，在猫科动物中，由 sp. 感染引起的中枢神经系统炎症疾病报道较少，在短尾猫中尚未有报道。在此，我们简要回顾了其他猫科动物相关的中枢神经系统疾病，并确认它也会影响短尾猫，同时强调了在常规诊断工作中对 sp. 进行种属鉴定的挑战。
