Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland.
Department of Periodontology and Operative Dentistry, University Medical Centre of the Johannes Gutenberg University, Mainz, Germany.
J Clin Periodontol. 2021 Apr;48(4):560-569. doi: 10.1111/jcpe.13426. Epub 2021 Feb 1.
To histologically evaluate the effect of a new collagen matrix on periodontal regeneration.
Two-wall intrabony defects were surgically created bilaterally distally to the maxillary first and third pre-molars in beagle dogs. The defects were randomly allocated to open flap debridement either with (test) or without (control) a volume-stable collagen matrix (VCMX). After 12 weeks, the dogs were euthanized, and the specimens histologically processed. Descriptive, histomorphometrical (vertical gain of periodontal tissues) and statistical analyses were then performed.
Healing was uneventful in most cases. Residual VCMX was still present and showed integration into new bone, new periodontal ligament, connective tissue and, in some specimens, into new cementum. Periodontal regeneration occurred to a varying extent in both groups. New continuous cementum and new bone formation were statistically significantly greater in the test group (4.12 mm and 3.28 mm, respectively) than in the control group (1.54 mm and 2.47 mm, respectively) (p = .009 and p = .037, respectively). The junctional epithelium was longer in the control group (2.21 mm) than in the test group (1.49 mm, p = .16).
The present results have for the first time provided histologic evidence for the potential of this novel VCMX to facilitate periodontal regeneration thus warranting further pre-clinical and clinical testing.
组织学评估新型胶原基质对牙周再生的效果。
在比格犬上颌第一和第三前磨牙的远中侧双侧手术制作双壁骨内缺损。将缺损随机分为翻瓣清创术组（实验组）和不使用体积稳定胶原基质（VCMX）的翻瓣清创术组（对照组）。12 周后，处死犬，进行标本组织学处理。然后进行描述性、组织形态计量学（牙周组织垂直增量）和统计学分析。
大多数情况下愈合顺利。残留的 VCMX 仍然存在，并整合到新骨、新牙周韧带、结缔组织中，在一些标本中还整合到新牙骨质中。两组均不同程度地发生牙周再生。实验组的新连续牙骨质和新骨形成明显多于对照组（分别为 4.12mm 和 3.28mm）（p=.009 和 p=.037）。对照组的结合上皮较长（2.21mm），实验组较短（1.49mm，p=.16）。
本研究首次提供了组织学证据，证明这种新型 VCMX 具有促进牙周再生的潜力，值得进一步进行临床前和临床研究。
