Engineering Research Center of Biomass Materials, Ministry of Education, School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621010, PR China.
Engineering Research Center of Biomass Materials, Ministry of Education, School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621010, PR China.
Int J Biol Macromol. 2024 Oct;278(Pt 3):134883. doi: 10.1016/j.ijbiomac.2024.134883. Epub 2024 Aug 19.
Uranium is a key element in the nuclear industry, whose accidental release causes health and environmental problems. In this paper, a protein-directed fluorescent sensor with aggregation-induced emission characteristics (gold nanoclusters@ovalbumin, AuNCs@OVA) was synthesized for the detection of UO with high sensitivity and selectivity. The sensor exhibited good fluorescence stability, and its fluorescence intensity could be selectively enhanced by UO. Based on FT-IR and XPS analyses, the increase in fluorescence intensity of AuNCs@OVA after the addition of UO was attributed to aggregation induced by the complexation between UO and the amino, carboxyl, hydroxyl, and phosphate groups of ovalbumin. The detection limit was determined to be 34.4 nM, and the sensor showed excellent ion selectivity for UO. In combination with a smartphone program, the sensor could realize the real-time detection of UO in a quantitative and portable way.
铀是核工业的关键元素，其意外释放会导致健康和环境问题。在本文中，我们合成了一种具有聚集诱导发射特性的蛋白导向荧光传感器（金纳米簇@卵清蛋白，AuNCs@OVA），用于高灵敏度和选择性地检测 UO。该传感器具有良好的荧光稳定性，其荧光强度可以被 UO 选择性增强。基于傅里叶变换红外光谱（FT-IR）和 X 射线光电子能谱（XPS）分析，加入 UO 后 AuNCs@OVA 荧光强度的增加归因于 UO 与卵清蛋白的氨基、羧基、羟基和磷酸基团之间的络合诱导聚集。检测限为 34.4 nM，该传感器对 UO 具有优异的离子选择性。结合智能手机程序，该传感器可以实现 UO 的实时定量便携检测。
