Universidade de São Paulo, Brazil.
Instituto Butantan, Brazil.
J Invertebr Pathol. 2024 Sep;206:108178. doi: 10.1016/j.jip.2024.108178. Epub 2024 Aug 20.
Because of the exoskeleton, arthropods must have flexible areas to be able to move. Such regions are called arthrodial membranes and are particularly vulnerable to bacteria and fungi. Here, we analyzed the secretion in the glands underneath it in a Neotropical harvester Mischonyx squalidus (Arachnida: Opiliones) and tested whether it has antiseptical properties. Wepuncturedthemembrane,collectedand quantified ina spectrophotometer. We also fractionated and analyzed the samples in reversed-phase high-performance liquid chromatography (RP-HPLC) and then incubated the treated fractions and determined growth inhibition by measuring absorbance. The secretions resulted in 100 fractions, among which two had activity against the Gram-positive bacteria Micrococcus luteus and against the yeast Candida albicans. The low concentrations at which the secretions were active are relevant from a biotechnological point of view. For the organism, the secretions possibly prevent infections, including when they are attacked in these regions by predators that pick that spot to bite.
由于外骨骼的存在，节肢动物必须有灵活的区域才能移动。这些区域被称为关节膜，特别容易受到细菌和真菌的侵害。在这里，我们分析了新热带收获者 Mischonyx squalidus（蛛形纲：蜘蛛目）下面腺体的分泌物，并测试了它是否具有防腐性能。我们刺破了膜，在分光光度计中收集并定量。我们还进行了反相高效液相色谱（RP-HPLC）的分级和分析，然后孵育处理的馏分，并通过测量吸光度来确定生长抑制。分泌物产生了 100 个馏分，其中两个馏分对革兰氏阳性菌微球菌和酵母白色念珠菌有活性。从生物技术的角度来看，分泌物在如此低的浓度下具有活性是相关的。对于该生物而言，分泌物可能会预防感染，包括当它们在这些区域受到以该部位为目标的捕食者攻击时。
