Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, Warsaw, 02-106, Poland.
Maria Curie-Skłodowska University, Marii Curie-Skłodowskiej 5, Lublin, 20-031, Poland.
Nat Commun. 2024 Aug 21;15(1):7188. doi: 10.1038/s41467-024-51654-6.
The transcriptional control of sporulation in Bacillus subtilis is reasonably well understood, but its translational control is underexplored. Here, we use RNA-seq, ribosome profiling and fluorescence microscopy to study the translational dynamics of B. subtilis sporulation. We identify two events of translation silencing and describe spatiotemporal changes in subcellular localization of ribosomes during sporulation. We investigate the potential regulatory role of ribosomes during sporulation using a strain lacking zinc-independent paralogs of three zinc-dependent ribosomal proteins (L31, L33 and S14). The mutant strain exhibits delayed sporulation, reduced germination efficiency, dysregulated translation of metabolic and sporulation-related genes, and disruptions in translation silencing, particularly in late sporulation.
枯草芽孢杆菌孢子形成的转录控制已经得到了很好的理解，但它的翻译控制还没有得到充分的研究。在这里，我们使用 RNA-seq、核糖体图谱和荧光显微镜来研究枯草芽孢杆菌孢子形成的翻译动力学。我们鉴定了两次翻译沉默事件，并描述了核糖体在孢子形成过程中的亚细胞定位的时空变化。我们使用一种缺乏锌依赖核糖体蛋白（L31、L33 和 S14）的锌非依赖性同工酶的菌株来研究核糖体在孢子形成过程中的潜在调节作用。突变株表现出孢子形成延迟、发芽效率降低、代谢和孢子形成相关基因的翻译失调，以及翻译沉默的破坏，特别是在晚期孢子形成过程中。
