用四氧化锇修饰大肠杆菌ColE1 DNA可产生正超螺旋分子。
Modification of ColE1 DNA with osmium tetroxide generates positively supercoiled molecules.
作者信息
Vojtísková M, Stokrová J, Palecek E
机构信息
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
出版信息
J Biomol Struct Dyn. 1985 Feb;2(5):1013-9. doi: 10.1080/07391102.1985.10507617.
Abstract
Covalent binding of osmium tetroxide to negatively supercoiled DNA in vitro initially induces its relaxation, accompanied by a formation of a single denaturation "bubble" per molecule. Binding of further osmium results in DNA overwinding and the appearance of positive supercoils as demonstrated by gel electrophoresis and electron microscopy.
摘要
在体外，四氧化锇与负超螺旋DNA的共价结合最初会使其松弛，同时每个分子形成一个单链变性“泡”。凝胶电泳和电子显微镜显示，进一步结合四氧化锇会导致DNA过度缠绕并出现正超螺旋。
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