Probing of DNA polymorphic structure in the cell with osmium tetroxide.
作者信息
Palecek E, Rasovská E, Boublíková P
机构信息
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
出版信息
Biochem Biophys Res Commun. 1988 Jan 29;150(2):731-8. doi: 10.1016/0006-291x(88)90452-4.
PMID:2829882
Abstract
It is shown that osmium tetroxide, 2,2'-bipyridine can be applied as a probe of DNA structure in a bacterial cell. Using this probe we demonstrate (a) presence of structural distortions at the junctions between the right-handed B and left-handed Z DNA in a recombinant plasmid pRW751 and (b) unusual structure of the d(A-T)16 insert in pAT32 plasmid in E. coli cells and in in vitro.
摘要
Zotero 插件