[Importance of cellular homogenization for the posterior isolation of plasma membranes of the Candida utilis yeast].

Candida utilis plasma membranes were isolated from different homogenized, either whole cells or spheroplasts, by centrifugation and aggregation of mitochondrial and internal membranes at pH 4.0. As judged by electron microscopy, chemical analysis, disc electrophoresis and enzymatic assays, membrane preparations with different degrees of purity were obtained. The purest plasma membranes were those prepared by homogenization of spheroplasts in the Omnimixer Sorvall followed aggregation at mitochondrial isoelectric point as described above.