Pérez Cabo A, Novaes-Ledieu M, García Mendoza C
Instituto de Microbiología, Jaime Ferrán, C.I.S.I.C., Madrid, España.
Microbiologia. 1985 Sep;1(1-2):19-27.
Candida utilis plasma membranes were isolated from different homogenized, either whole cells or spheroplasts, by centrifugation and aggregation of mitochondrial and internal membranes at pH 4.0. As judged by electron microscopy, chemical analysis, disc electrophoresis and enzymatic assays, membrane preparations with different degrees of purity were obtained. The purest plasma membranes were those prepared by homogenization of spheroplasts in the Omnimixer Sorvall followed aggregation at mitochondrial isoelectric point as described above.
产朊假丝酵母的质膜是通过在pH 4.0条件下，对不同匀浆的全细胞或原生质球进行离心，使线粒体膜和内膜聚集而分离得到的。通过电子显微镜、化学分析、圆盘电泳和酶活性测定判断，获得了不同纯度的膜制剂。最纯的质膜是按照上述方法，将原生质球在Omnimixer Sorvall中匀浆，然后在线粒体等电点进行聚集制备得到的。
