Pero R W, Osterman-Golkar S, Högstedt B
Wallenberg Laboratory, Molecular Ecogenetics, University of Lund, Sweden.
Cell Biol Toxicol. 1985 Oct;1(4):309-14. doi: 10.1007/BF00118195.
Exposure to propylene oxide was determined previously by the degree of alkylation of hemoglobin measured on the histidine residue as N-3-(2-hydroxypropyl) histidine, using blood samples from 8 propylene oxide-exposed employees and 13 unexposed referents. Mononuclear leukocytes isolated from the same blood samples were used to quantify DNA repair proficiency following an in vitro challenge with the carcinogen, N-acetoxy-2-acetylamino-fluorene. Decreases in the DNA repair proficiency index correlated significantly to in vivo exposure levels to propylene oxide (r = -0.64, p less than 0.03). These data suggest a possible short-term biological assay for monitoring the in vivo genotoxic effects of propylene oxide exposure in the human population.
先前通过测量血红蛋白在组氨酸残基上烷基化程度来确定环氧丙烷暴露情况，该烷基化产物为N - 3 -（2 - 羟丙基）组氨酸，使用了8名环氧丙烷暴露员工和13名未暴露对照者的血样。从相同血样中分离出的单核白细胞用于在用致癌物N - 乙酰氧基 - 2 - 乙酰氨基芴进行体外攻击后量化DNA修复能力。DNA修复能力指数的降低与环氧丙烷的体内暴露水平显著相关（r = -0.64，p小于0.03）。这些数据表明可能存在一种短期生物学检测方法，用于监测人群中环氧丙烷暴露的体内遗传毒性效应。
