Sultan Amira M, Mahmoud Noha Mostafa
MD, Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, 35516 Elgomhouria S, Mansoura, Egypt.
MD, Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, 35516 Elgomhouria S, Mansoura, Egypt, and Medical Microbiology and Immunology Department, Faculty of Medicine, Horus University, New Damietta, Egypt.
Germs. 2024 Mar 31;14(1):11-19. doi: 10.18683/germs.2024.1413. eCollection 2024 Mar.
Integrons are genetic systems that may confer antibiotic resistance to . Biofilm formation can facilitate gene exchange and can accelerate the development of antibiotic resistance. The aim of this work was to assess the distribution of resistance integrons including class 1, 2 and 3 among biofilm- and non-biofilm producing clinical strains of We also aimed to investigate the relationship between the existence of these integrons and the isolates' resistance patterns.
Specimens were obtained from patients showing evidence of infection. isolates were identified using conventional techniques, while disk diffusion test was used to detect their antimicrobial susceptibilities. Biofilm formation was detected by the tissue culture plate technique, while classes of integrons were detected by polymerase chain reaction.
Out of 106 isolates, 55.7% were class 1 integron-positive while 19.8% were class 2 integron-positive. However, class 3 integrons were not detected. Significant associations were found between class 1 integrons and resistance toward amikacin, gentamicin, cefepime, ceftazidime and ciprofloxacin. Class 2 integrons were associated with amikacin, ceftazidime and cefepime resistance. Of pseudomonal isolates, 61.3% were biofilm producing. Biofilm production was associated significantly with the existence of class 1 integrons (p<0.001) and class 2 integrons (p=0.039).
About two thirds of isolated strains harbored resistance integrons, which emphasized their significance in our locality. The frequencies of class 1 and 2 integrons were significantly higher among biofilm forming isolates. Ongoing surveillance and infection control strategies are necessary to limit spread of integrons.
整合子是一种遗传系统，可赋予对……的抗生素抗性。生物膜形成可促进基因交换并加速抗生素抗性的发展。本研究的目的是评估1类、2类和3类抗性整合子在生物膜产生菌和非生物膜产生菌临床菌株中的分布情况。我们还旨在研究这些整合子的存在与分离株抗性模式之间的关系。
从有感染迹象的患者中获取标本。使用传统技术鉴定菌株，同时采用纸片扩散法检测其抗菌敏感性。通过组织培养板技术检测生物膜形成，而通过聚合酶链反应检测整合子类别。
在106株菌株中，55.7%为1类整合子阳性，19.8%为2类整合子阳性。然而，未检测到3类整合子。发现1类整合子与对阿米卡星、庆大霉素、头孢吡肟、头孢他啶和环丙沙星的抗性之间存在显著关联。2类整合子与对阿米卡星、头孢他啶和头孢吡肟的抗性相关。在假单胞菌分离株中，61.3%产生生物膜。生物膜产生与1类整合子（p<0.001）和2类整合子（p=0.039）的存在显著相关。
约三分之二的分离菌株携带抗性整合子，这突出了它们在我们当地的重要性。在形成生物膜的分离株中，1类和2类整合子的频率显著更高。持续的监测和感染控制策略对于限制整合子的传播是必要的。
