从伊朗北部一家烧伤中心废水中分离出的铜绿假单胞菌菌株的抗生素耐药性及1类整合子频率

Antibiotic resistance and frequency of class 1 integrons among Pseudomonas aeruginosa isolates obtained from wastewaters of a burn center in Northern Iran.

作者信息

Ebrahimpour M, Nikokar I, Ghasemi Y, Sedigh Ebrahim-Saraie H, Araghian A, Farahbakhsh M, Ghassabi F

机构信息

Department of Biotechnology, Laboratory of Microbiology and Immunology of Infectious Diseases, Para Medicine Faculty, Guilan University of Medical Sciences, Rasht, Iran.

Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

出版信息

Ann Ig. 2018 Mar-Apr;30(2):112-119. doi: 10.7416/ai.2018.2202.

DOI:10.7416/ai.2018.2202

PMID:29465148

Abstract

BACKGROUND

Pseudomonas aeruginosa, being responsible of a broad variety of infections, is considered an important nosocomial pathogen. The emergence of multiple-drug resistance among strains of P. aeruginosa appeared as a further public health concern. Due to the considerable ability of multiple-drug resistant P. aeruginosa strains to transmit themselves in the environment, we aimed to investigate the association of class 1 integrons with the antibiotic resistance profile of P. aeruginosa strains isolated from hospital wastewaters.

METHODS

In this cross-sectional study, a total of 100 P. aeruginosa isolates were obtained from raw wastewater samples from February 2010 to January 2011 in a Teaching Burn Hospital in Guilan province. All isolates were identified as P. aeruginosa using standard microbiological tests. Antibiotic susceptibility was investigated using the disk diffusion method according to Clinical and Laboratory Standards Institute recommendations. All isolates were assayed for the presence of the class 1 integrons gene by PCR.

RESULTS

Overall, 30 (30%) P. aeruginosa isolates were positive for the presence of class 1 integrons. The highest antibiotic resistance rates in both integron-positive and -negative isolates belonged to cephalexin and cephazolin, with 100% resistance. Amikacin and ciprofloxacin with the lowest level of resistance (13.3%) were the effective antibiotics against integron-positive isolates. The rates of MDR isolates were significantly higher among integron-positive isolates with 43.3% compared to negative isolates with 22.9% (P < 0.05).

CONCLUSION

The results highlight the importance of class 1 integrons in multiple antibiotic resistance among P. aeruginosa isolates. Moreover, the spread of hospital derived wastewaters in the environment can be regarded as the origin of significant reservoirs for antibiotic-resistant pathogens.

摘要

背景

铜绿假单胞菌可引发多种感染，被视为一种重要的医院病原体。铜绿假单胞菌菌株中多重耐药性的出现引发了进一步的公共卫生问题。鉴于多重耐药铜绿假单胞菌菌株在环境中有很强的传播能力，我们旨在研究1类整合子与从医院废水中分离出的铜绿假单胞菌菌株抗生素耐药谱之间的关联。

方法

在这项横断面研究中，2010年2月至2011年1月期间，从吉兰省一家烧伤教学医院的原废水样本中总共获得了100株铜绿假单胞菌分离株。使用标准微生物学检测方法将所有分离株鉴定为铜绿假单胞菌。根据临床和实验室标准协会的建议，采用纸片扩散法检测抗生素敏感性。通过聚合酶链反应（PCR）检测所有分离株中1类整合子基因的存在情况。

结果

总体而言，30株（30%）铜绿假单胞菌分离株的1类整合子呈阳性。整合子阳性和阴性分离株中抗生素耐药率最高的是头孢氨苄和头孢唑林，耐药率均为100%。阿米卡星和环丙沙星耐药水平最低（13.3%），是针对整合子阳性分离株的有效抗生素。整合子阳性分离株中的多重耐药分离株率显著高于阴性分离株，分别为43.3%和22.9%（P<0.05）。