Budhathoki Sujan, Chaudhary Nabina, Guragain Biswash, Baral Dipak, Adhikari Janak, Chaudhary Narendra Kumar
Department of Chemistry, Mahendra Morang Adarsh Multiple Campus, Biratnagar, Tribhuvan University, Nepal.
Department of Intensive Care Unit, Birat Medical College and Teaching Hospital, Biratnagar, Nepal.
Heliyon. 2024 Aug 3;10(15):e35642. doi: 10.1016/j.heliyon.2024.e35642. eCollection 2024 Aug 15.
Plant-mediated synthesis of silver nanoparticles (AgNPs) is an eco-friendly and convenient alternative to conventional methods. leaf extract (BHE) was used in this study to reduce metal salts and cap and stabilize nanoparticles (NPs), which were characterized and tested for antibacterial and anti-corrosion properties. Stirring the extract with AgNO led to a color change, indicating nanoparticle formation. The absorption peak at 428 nm in the UV-visible spectrum further validated its formation. The AgNPs were characterized using various techniques such as FTIR, UV-visible, PXRD, HRTEM, SEM, and EDX. Powder X-ray diffraction analysis confirmed its nanocrystalline nature, with an average crystallite size of 17.92 nm. The FTIR spectrum showed hydroxyl, amine, amide, and carbonyl groups as capping and reducing agents for the AgNPs. SEM analysis revealed poly-dispersed NPs of various sizes, while EDX showed an intense peak for Ag, and TEM images revealed mostly hexagonal and triangular NPs. Antibacterial activity was tested against three human pathogens: Significant antibacterial activity was observed specifically against , with an 11 mm inhibition zone. Both plant extracts and AgNPs inhibited acid-induced corrosion, with the highest inhibition efficiencies of 81.69 % and 69.54 % at 1000 ppm, respectively. With rising concerns over bacterial resistance and metal corrosion, this study addresses global challenges related to new antimicrobial agents, which are crucial for combating antibiotic resistance and protecting metals in various industries.
植物介导的银纳米颗粒(AgNPs)合成是一种比传统方法更环保、更便捷的替代方法。本研究使用了叶片提取物(BHE)来还原金属盐并包覆和稳定纳米颗粒(NPs),对其进行了表征,并测试了抗菌和防腐性能。将提取物与硝酸银搅拌会导致颜色变化,表明纳米颗粒形成。紫外可见光谱中428nm处的吸收峰进一步证实了其形成。使用傅里叶变换红外光谱(FTIR)、紫外可见光谱、粉末X射线衍射(PXRD)、高分辨率透射电子显微镜(HRTEM)、扫描电子显微镜(SEM)和能量散射X射线光谱(EDX)等多种技术对AgNPs进行了表征。粉末X射线衍射分析证实了其纳米晶性质,平均晶粒尺寸为17.92nm。FTIR光谱显示羟基、胺基、酰胺基和羰基作为AgNPs的包覆和还原剂。SEM分析揭示了各种尺寸的多分散NPs,而EDX显示Ag有一个强峰,TEM图像显示大多为六边形和三角形NPs。针对三种人类病原体测试了抗菌活性:特别观察到对[病原体名称未给出]有显著的抗菌活性,抑菌圈为11mm。植物提取物和AgNPs都抑制了酸诱导的腐蚀,在1000ppm时最高抑制效率分别为81.69%和69.54%。随着对细菌耐药性和金属腐蚀的担忧加剧,本研究应对了与新型抗菌剂相关的全球挑战,这些抗菌剂对于对抗抗生素耐药性和保护各行业中的金属至关重要。