Zhang Yishan, Liu Fanghong, Mo Dan, Jiang Yiling, Lin Tian, Deng Sicheng, Lan Jue, Qiu Rongmin
College & Hospital of Stomatology, Guangxi Medical University, Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Key Laboratory of Research and Application of Stomatological Equipment, Education Department of Guangxi Zhuang Autonomous Region, No.10 Shuangyong Road, Nanning, Guangxi, 530021, China.
Heliyon. 2024 Aug 3;10(15):e35238. doi: 10.1016/j.heliyon.2024.e35238. eCollection 2024 Aug 15.
The primary objective of this study was to scrutinise the disparities in the diversity, structure, and function of the oral microbiome among caries-free children from the Zhuang and Han ethnic groups with a focus on the influence of ethnically distinct oral health behaviours on the composition of the oral microbiota.
A questionnaire survey was conducted to assess oral health behaviours and dental plaque samples were collected from 96 Zhuang and Han children aged 4-5 years living in Guangxi, southern China for high-throughput sequencing. PCR amplification was performed for sequencing of the V4 region of the 16S rDNA gene, and second-generation sequencing was performed using the Illumina MiSeq platform to compare and analyse the diversity, structure and function of the microbiota.
Single-factor analysis revealed significant differences between the Zhuang and Han ethnic groups regarding juice consumption, the frequency of consuming sugar-sweetened food or beverages before bedtime, the age that individuals started toothbrushing, the frequency of toothbrushing and the frequency of parental assistance with toothbrushing ( < 0.001). The dominant phyla were , etc., and the dominant genera included and . The dental plaques of the caries-free Zhuang and Han ethnic groups had similar core microbiomes, with no significant differences in the diversity and structure of the microbiota and no significant differences in the abundance of the dominant genera. In addition, no significant difference in metabolic function was observed between the Zhuang and Han ethnic groups.
The core oral microbiota was consistent in caries-free Zhuang and Han children. Despite differences in dietary habits and oral hygiene behaviours between the Zhuang and Han ethnic groups, with a high frequency of sugary food intake but better oral health behaviours in the Zhuang group, there were no significant differences in the diversity, structure and function of the oral microbiota of caries-free children in the Zhuang and Han ethnic groups.
本研究的主要目的是审视壮族和汉族无龋儿童口腔微生物群在多样性、结构和功能上的差异，重点关注不同民族的口腔健康行为对口腔微生物群组成的影响。
进行问卷调查以评估口腔健康行为，并从中国南方广西的96名4 - 5岁壮族和汉族儿童中收集牙菌斑样本进行高通量测序。对16S rDNA基因的V4区域进行PCR扩增测序，并使用Illumina MiSeq平台进行第二代测序，以比较和分析微生物群的多样性、结构和功能。
单因素分析显示，壮族和汉族在果汁饮用、睡前食用含糖食物或饮料的频率、开始刷牙的年龄、刷牙频率以及父母协助刷牙的频率方面存在显著差异（<0.001）。优势菌门为等，优势菌属包括和。无龋壮族和汉族儿童的牙菌斑具有相似的核心微生物群，微生物群的多样性和结构无显著差异，优势菌属的丰度也无显著差异。此外，壮族和汉族在代谢功能方面未观察到显著差异。
无龋壮族和汉族儿童的核心口腔微生物群是一致的。尽管壮族和汉族在饮食习惯和口腔卫生行为上存在差异，壮族含糖食物摄入频率高但口腔健康行为较好，但无龋壮族和汉族儿童口腔微生物群在多样性、结构和功能上无显著差异。
