Li Zunyan, Hu Yufeng, Li Haibin, Lin Yingting, Cheng Ming, Zhu Fenghua, Guo Yixuan
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Qingdao Animal Husbandry and Veterinary Research Institute, Qingdao, China.
Front Vet Sci. 2024 Aug 7;11:1447238. doi: 10.3389/fvets.2024.1447238. eCollection 2024.
The effects of yeast culture (YC) on dairy goat milk yield and potential effects of rumen microbial population changes on rumen fermentation are poorly understood. This study aimed to evaluate the effects of YC on milk yield and rumen fermentation in dairy goats and explore the potential microbial mechanisms. Forty Laoshan dairy goats with a weight of 51.23 ± 2.23 kg and daily milk yield of 1.41 ± 0.26 kg were randomly divided into 4 groups: control (no YC), YC1 (10 g/day per goat), YC2 (25 g/day per goat), and YC3 (40 g/day per goat). The pre-feeding period was 15 days, and the official period was 60 days. Laoshan dairy goats were milked twice daily, and the individual milk yield was recorded. On the last day of the official period, rumen fluid was collected to measure rumen fermentation, perform quantitative polymerase chain reaction (PCR), and detect metabolites. Compared to the control group, the YC group had greater milk yield; higher acetic acid, butyric acid, and total volatile fatty acid contents; and lower ammonia-N (NH-N) content in the rumen ( < 0.05). YC increased the abundance of and ( < 0.05). Differential metabolites L-leucine and aspartic acid were screened. This study revealed the microbial mechanisms linking the relative abundance of and to L-leucine and aspartic acid utilization. These results describe the potential benefits of supplementing 10 g/day per goat YC in the diets of Laoshan dairy goats for improving the rumen environment and milk yield.
酵母培养物（YC）对奶山羊产奶量的影响以及瘤胃微生物种群变化对瘤胃发酵的潜在影响尚不清楚。本研究旨在评估YC对奶山羊产奶量和瘤胃发酵的影响，并探索潜在的微生物机制。将40只体重为51.23±2.23千克、日产奶量为1.41±0.26千克的崂山奶山羊随机分为4组：对照组（不添加YC）、YC1组（每只山羊每天10克）、YC2组（每只山羊每天25克）和YC3组（每只山羊每天40克）。预饲期为15天，正式期为60天。崂山奶山羊每天挤奶两次，并记录个体产奶量。在正式期的最后一天，采集瘤胃液以测量瘤胃发酵、进行定量聚合酶链反应（PCR）并检测代谢产物。与对照组相比，YC组产奶量更高；瘤胃中乙酸、丁酸和总挥发性脂肪酸含量更高；氨氮（NH-N）含量更低（P<0.05）。YC增加了[具体微生物名称1]和[具体微生物名称2]的丰度（P<0.05）。筛选出差异代谢产物L-亮氨酸和天冬氨酸。本研究揭示了[具体微生物名称1]和[具体微生物名称2]的相对丰度与L-亮氨酸和天冬氨酸利用之间的微生物机制。这些结果描述了在崂山奶山羊日粮中每只山羊每天补充10克YC对改善瘤胃环境和产奶量的潜在益处。
