Department of Midwifery, Zubeyde Hanım Faculty of Health Sciences, Nigde Omer Halisdemir University, Nigde, Turkey.
Niğde Ömer Halisdemir Üniversitesi, Derbent Yerleşkesi, Atatürk Bulvarı, Merkez, Nigde, 51200, Turkey.
J Mol Histol. 2024 Oct;55(5):1009-1019. doi: 10.1007/s10735-024-10248-8. Epub 2024 Aug 22.
An actin binding protein, gelsolin (GSN) has two isoforms, plasma (pGSN) and cytosolic (cGSN). Changes in pGSN and/or cGSN levels have been shown to be associated with the pathogenesis of several diseases. The aim of this study was to evaluate changes in intracellular and extracellular GSNlevels with HIF-1 in animals exposed to chronic sustained hypoxia (CSH), in addition to apoptosis and the cellular redox status. The rats in the Sham group were exposed to 21% O, and the rats in the hypoxia groups were exposed to 13 and 10% O, respectively. Plasma pGSN, HIF-1α, Total Antioxidant Status (TAS) and Total Oxidant Status (TOS), and lung tissue pGSN, HIF-1α, TAS, TOS, GSN levels, and apoptotic cell numbers were measured. HIF-1α levels were found to increase significantly in the tissue, especially in the group with severe hypoxia, both in biochemical and histological examinations. pGSN levels were also significantly decreased in both plasma and tissue. Significant increases in tissue were observed in cGSN. It was observed that while the antioxidant activity was dominant in the tissue, the oxidant activity was dominant in the plasma. In particular, the response to hypoxia regulated by HIF-1 is very important for cellular survival. The results of this study showed that the increase in cGSN and TAS levels in the lung tissue together with HIF-1α can be considered as the activation of mechanisms for cellular protection.
肌动蛋白结合蛋白，即凝胶蛋白（GSN）有两种亚型，即血浆型（pGSN）和胞浆型（cGSN）。已有研究表明，pGSN 和/或 cGSN 水平的变化与多种疾病的发病机制有关。本研究旨在评估在慢性持续低氧（CSH）暴露的动物中，细胞内和细胞外 GSN 水平的变化与 HIF-1 以及细胞凋亡和细胞氧化还原状态的关系。假手术组的大鼠暴露于 21%的 O 中，而低氧组的大鼠分别暴露于 13%和 10%的 O 中。测量了血浆 pGSN、HIF-1α、总抗氧化状态（TAS）和总氧化状态（TOS）以及肺组织 pGSN、HIF-1α、TAS、TOS、GSN 水平和凋亡细胞数量。在组织中，特别是在严重低氧组的生化和组织学检查中，HIF-1α 水平显著增加。在血浆和组织中，pGSN 水平也显著降低。在组织中，cGSN 水平显著增加。观察到，尽管组织中的抗氧化活性占主导地位，但血浆中的氧化活性占主导地位。特别是，HIF-1 调节的低氧反应对细胞存活非常重要。本研究结果表明，肺组织中 cGSN 和 TAS 水平的增加以及 HIF-1α 的增加可以被认为是细胞保护机制的激活。
