Institute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, P. R. China.
Anal Chem. 2024 Sep 3;96(35):14291-14297. doi: 10.1021/acs.analchem.4c03273. Epub 2024 Aug 22.
The mitochondria, as one of the essential organelles in cells, are closely associated with numerous biological processes. Therefore, the realization of clear and real-time imaging for tracking mitochondria is of profound significance. Here, we present a mitochondria-targeting fluorescent probe, , for the real-time fluorescence imaging of mitochondria in living cells. Using the probe, the fluorescence changes of mitochondria stimulated by different drugs were successfully observed by fluorescence imaging. In addition, the dynamic processes of mitochondria and lysosomes during apoptosis were also explored. Importantly, we observed several novel dynamic interaction patterns between mitochondria and lysosomes. Among them, the most prominent pattern involved the noncontact movements of two lysosomes, that is, one lysosome gradually approached the other lysosome over time, eventually coming into contact and merging with it while gradually combining with mitochondria to form new mitochondria. Notably, the protrusions of the mitochondria became increasingly evident during this process. Meanwhile, we successfully observed the dynamic changes of mitochondria with SIM super-resolution imaging. The study provides promising help for the in-depth study of the dynamic processes of mitochondrial physiology and pathology and the study of the interactions between organelles.
线粒体作为细胞内的重要细胞器之一，与许多生物过程密切相关。因此，实现对线粒体的清晰实时成像追踪具有重要意义。在这里，我们提出了一种线粒体靶向荧光探针 ，用于活细胞中线粒体的实时荧光成像。使用该探针，我们通过荧光成像成功观察到了不同药物刺激下线粒体的荧光变化。此外，还探索了细胞凋亡过程中线粒体和溶酶体的动态过程。重要的是，我们观察到了线粒体和溶酶体之间几种新的动态相互作用模式。其中，最显著的模式涉及两个溶酶体的非接触运动，即一个溶酶体随着时间的推移逐渐接近另一个溶酶体，最终接触并融合在一起，同时逐渐与线粒体结合形成新的线粒体。值得注意的是，在此过程中，线粒体的突起变得越来越明显。同时，我们还成功地通过 SIM 超分辨率成像观察到了线粒体的动态变化。该研究为深入研究线粒体生理学和病理学的动态过程以及细胞器之间的相互作用提供了有前景的帮助。
