具有酸度非依赖性溶酶体保留机制的双功能超分辨率成像探针。
Bifunctional Super-resolution Imaging Probe with Acidity-Independent Lysosome-Retention Mechanism.
机构信息
Department of Chemical Biology, College of Chemistry and Chemical Engineering, State Key Laboratory for Physical Chemistry of Solid Surfaces, the Key Laboratory for Chemical Biology of Fujian Province, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, and Innovation Center for Cell Signaling Network , Xiamen University , Xiamen , 361005 , China.
State key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences , Xiamen University , Xiamen , 361005 , China.
出版信息
Anal Chem. 2018 Oct 2;90(19):11393-11400. doi: 10.1021/acs.analchem.8b02365. Epub 2018 Sep 10.
Spatiotemporal imaging is of enormous use to explore organelle biology, necessitating organelle-tracing techniques reliable in varied cell stress. We herein reported lysosomal imaging using rhodamine-X-integrated sialic acid (SA), which is stably maintained in lysosomes irrespective of lysosomal pH changes. Exhibiting bright fluorescence and superior photostability, SA enables 120 h continual tracking of fusion/fission of lysosomes and mitochondrion-lysosome interaction in mitophagy. Relative to conventional acidotropic probes prone to dissipation from stressed lysosomes, SA offers a new route for long-term tracking of stressed lysosomes relevant to diverse pathological conditions.
时空成像对于探索细胞器生物学具有巨大的作用，这就需要细胞器追踪技术在不同的细胞应激条件下可靠。在此，我们报告了使用整合了罗丹明 X 的唾液酸（SA）进行溶酶体成像的方法，无论溶酶体 pH 值如何变化，SA 都能稳定地存在于溶酶体中。SA 具有明亮的荧光和优异的光稳定性，可实现溶酶体融合/裂变以及线粒体-溶酶体相互作用在细胞自噬中的 120 小时持续追踪。与易从应激溶酶体中耗散的传统酸敏探针相比，SA 为长期追踪与多种病理状况相关的应激溶酶体提供了一种新途径。
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