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School of biological and food processing engineering, Huanghuai University, Zhumadian, Henan province 463000, PR China.
School of biological and food processing engineering, Huanghuai University, Zhumadian, Henan province 463000, PR China; Zhumadian Academy of Industry Innovation and Development, Zhumadian, Henan province 463000, PR China.
Reprod Biol. 2024 Dec;24(4):100930. doi: 10.1016/j.repbio.2024.100930. Epub 2024 Aug 21.
Effect of resveratrol (RSV) on spermatogenesis and the mechanism of resveratrol in promoting spermatogenesis of breeding boars was explored by feeding sexually mature Duroc boars with normal diet and 20 mg/kg resveratrol diet for 14 days to the control group and experimental group, respectively. Semen volume, sperm density, motility, viability and abnormality rate were analyzed on day 0, 7, and 14. Blood samples were collected, and levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T) in serum were analyzed. On day 14, the testis tissue was collected for antioxidant and proteomics analysis etc. The semen volume, sperm density, motility, and viability of the experimental group and the contents of serum FSH, LH, T and plasma SOD activity were significantly higher than those in the control group. However, the serum IL-6, TNF-α and plasma MDA were remarkably lower in experimental group. The above results showed that resveratrol can simulate spermatogenesis in breeding boars. Proteomic results demonstrated that three differentially expressed proteins (DEPs) were up-regulated and 12 DEPs were down-regulated; ODF1, calmodulin, Cabs1, and Hp were involved in spermatogenesis; and the main enriched metabolic pathway is steroid hormone synthesis pathway. Therefore, the improvement in sperm quality by resveratrol may be achieved by regulating the changes in outer dense fiber 1, calmodulin, spermatid specific 1, and haptoglobin expression and steroid synthesis pathway.
通过分别给性成熟的杜洛克公猪喂食正常日粮和含20mg/kg白藜芦醇的日粮14天，将其分为对照组和实验组，探讨白藜芦醇（RSV）对种公猪精子发生的影响及促进精子发生的机制。在第0、7和14天分析精液体积、精子密度、活力、存活率和畸形率。采集血样，分析血清中促卵泡激素（FSH）、促黄体生成素（LH）和睾酮（T）的水平。在第14天，采集睾丸组织进行抗氧化和蛋白质组学分析等。实验组的精液体积、精子密度、活力和存活率以及血清FSH、LH、T含量和血浆超氧化物歧化酶（SOD）活性均显著高于对照组。然而，实验组血清白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和血浆丙二醛（MDA）显著降低。上述结果表明，白藜芦醇可促进种公猪精子发生。蛋白质组学结果显示，有3种差异表达蛋白（DEPs）上调，12种DEPs下调；外致密纤维蛋白1（ODF1）、钙调蛋白、精子特异性蛋白1（Cabs1）和触珠蛋白（Hp）参与精子发生；主要富集的代谢途径是类固醇激素合成途径。因此，白藜芦醇对精子质量的改善可能是通过调节外致密纤维1、钙调蛋白、精子特异性蛋白1和触珠蛋白的表达变化以及类固醇合成途径来实现的。
