China Tobacco Guangdong Industrial Co., Ltd, Guangzhou, 510385, China.
Carbohydr Res. 2024 Oct;544:109226. doi: 10.1016/j.carres.2024.109226. Epub 2024 Jul 31.
Fig fruit is widely accepted in warm and tropical regions due to the sweet taste and health benefits. The polysaccharides in fig fruit are responsible for the health benefits. However, information regarding polysaccharide structure remains limited. In this study, the water-soluble polysaccharides (FFP) were extracted from fig fruit with a yield of 15.3 mg/g. Anion exchange chromatography and gel permeation chromatography were used for purification. A leading polysaccharide was purified and characterized as below. The monosaccharide composition of FFP included arabinose (Ara), galactose (Gal), galacturonic acid (GalA) and rhamnose (Rha). The glycosidic linkages were revealed to be L-Ara-(1→, →5)-L-Ara-(1→, →2,4)-L-Rha-(1→, →2)-L-Rha-(1→, →4)-D-Gal-(1→ and D-Gal-(1 → . Nuclear magnetic resonnance (NMR) spectra revealed that FFP had →4)-α-D-GalpA-(1 → 2)-α-L-Rhap-(1→ as backbone. The side chains included Galp-β-D-(1 → 4)-Galp-β-D-(1 → 4)-Galp-β-D-(1 → 4)-Galp-β-D-(1→ and α-L-Araf-(1 → 5)-α-L-Araf-(1 → . They were linked to C-4 of Rhap. FFP inhibited the production of NO, IL-6 and IL-1β levels induced by LPS. IL-6 and IL-1β levels were returned to normal. These information are helpful to understand this functional fruit.
由于甜美的口感和对健康的益处，无花果树广泛存在于温暖和热带地区。无花果中的多糖是其对健康有益的原因。然而，关于多糖结构的信息仍然有限。在这项研究中，从无花果中提取了水溶性多糖（FFP），得率为 15.3mg/g。采用阴离子交换色谱和凝胶渗透色谱进行纯化。一种主要的多糖被分离和鉴定如下。FFP 的单糖组成包括阿拉伯糖（Ara）、半乳糖（Gal）、半乳糖醛酸（GalA）和鼠李糖（Rha）。糖苷键被揭示为 L-Ara-(1→, →5)-L-Ara-(1→, →2,4)-L-Rha-(1→, →2)-L-Rha-(1→, →4)-D-Gal-(1→ 和 D-Gal-(1→。核磁共振（NMR）谱表明，FFP 具有→4)-α-D-GalpA-(1→2)-α-L-Rhap-(1→作为主链。侧链包括 Galp-β-D-(1→4)-Galp-β-D-(1→4)-Galp-β-D-(1→4)-Galp-β-D-(1→和 α-L-Araf-(1→5)-α-L-Araf-(1→。它们连接到 Rhap 的 C-4 上。FFP 抑制了 LPS 诱导的 NO、IL-6 和 IL-1β 水平的产生。IL-6 和 IL-1β 水平恢复正常。这些信息有助于理解这种功能性水果。
