Zhou Yan, Wang Shengchao, Feng Weisheng, Zhang Zhenling, Li Hongwei
College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China; Department of Traditional Chinese Medicine, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China.
College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Int J Biol Macromol. 2021 Sep 1;186:385-395. doi: 10.1016/j.ijbiomac.2021.06.100. Epub 2021 Jun 29.
The structures and immunomodulatory activities of two polysaccharides (SDH-WA and SDH-0.2A) from Rehmanniae Radix Praeparata (RRP) were investigated. RRP crude polysaccharide was obtained by water extraction and purified. Ion chromatography, high-performance gel permeation chromatography, Fourier-transform infrared spectroscopy, methylation analysis, gas chromatography-mass spectrometry, and nuclear magnetic resonance were used to characterize the polysaccharides. The main chain of SDH-WA was →6)-α-D-Galp-(1→6)-α-D-Galp-(1→5)-α-L-Araf-(1→3,5)-α-L-Araf-(1→, terminal sugar residue α-L-Araf-(1→ linked to residue →3,5)-α-L-Araf-(1→ on the main chain by an O-3 bond. The other two terminal sugar residues α-D-Galp-(1→ and →6)-β-D-Galp were linked to the end of the main chain. The main chain of SDH-0.2A was →2,4)-α-L-Rhap-(1→4)-α-D-GalpA-(1→. Three branched chains α-D-Galp-(1→6)-α-D-Galp-(1→5)-α-L-Araf-(1→3,5)-α-L-Araf-(1→, →3,6)-β-D-Galp-(1→5)-α-L-Araf-(1→, and →4)-β-D-Galp-(1→5)-α-L-Araf-(1→ were linked to the main chain residue →2,4)-α-L-Rhap-(1→ by an O-2 bond. Three terminal sugar residues α-D-Galp-(1→, α-L-Araf-(1→, and →6)-β-D-Galp were linked to the end of the chain. Both polysaccharides showed no cytotoxic effects on and significantly promoted the phagocytic activity of RAW264.7 cells. They dose-dependently improved lysozyme activity and stimulated the production of TNF-α and IL-6 by RAW264.7 cells, but attenuated the secretion of lysozymes, TNF-α, IL-6, IL-1β, and nitric oxide by lipopolysaccharide-induced RAW264.7 cells. The present studies suggest that PRR polysaccharide is a valuable source with immunomodulating.
研究了熟地黄中两种多糖（SDH-WA和SDH-0.2A）的结构和免疫调节活性。通过水提取和纯化得到熟地黄粗多糖。采用离子色谱、高效凝胶渗透色谱、傅里叶变换红外光谱、甲基化分析、气相色谱-质谱联用和核磁共振对多糖进行表征。SDH-WA的主链为→6)-α-D-吡喃半乳糖-(1→6)-α-D-吡喃半乳糖-(1→5)-α-L-阿拉伯呋喃糖-(1→3,5)-α-L-阿拉伯呋喃糖-(1→，末端糖残基α-L-阿拉伯呋喃糖-(1→通过O-3键与主链上的残基→3,5)-α-L-阿拉伯呋喃糖-(1→相连。另外两个末端糖残基α-D-吡喃半乳糖-(1→和→6)-β-D-吡喃半乳糖连接在主链末端。SDH-0.2A的主链为→2,4)-α-L-鼠李吡喃糖-(1→4)-α-D-吡喃半乳糖醛酸-(1→。三条支链α-D-吡喃半乳糖-(1→6)-α-D-吡喃半乳糖-(1→5)-α-L-阿拉伯呋喃糖-(1→3,5)-α-L-阿拉伯呋喃糖-(1→、→3,6)-β-D-吡喃半乳糖-(1→5)-α-L-阿拉伯呋喃糖-(1→和→4)-β-D-吡喃半乳糖-(1→5)-α-L-阿拉伯呋喃糖-(1→通过O-2键与主链残基→2,4)-α-L-鼠李吡喃糖-(1→相连。三个末端糖残基α-D-吡喃半乳糖-(1→、α-L-阿拉伯呋喃糖-(1→和→6)-β-D-吡喃半乳糖连接在链的末端。两种多糖对RAW264.7细胞均无细胞毒性作用，且显著促进其吞噬活性。它们剂量依赖性地提高溶菌酶活性，刺激RAW264.7细胞产生TNF-α和IL-6，但减弱脂多糖诱导的RAW264.7细胞中溶菌酶、TNF-α、IL-6、IL-1β和一氧化氮的分泌。本研究表明熟地黄多糖是一种具有免疫调节作用的宝贵来源。
