Department of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.
Department of Histology and Cell Biology, Faculty of Medicine, Menoufia University, Shebin Elkom 3251, Egypt.
Pathol Res Pract. 2024 Oct;262:155536. doi: 10.1016/j.prp.2024.155536. Epub 2024 Aug 12.
microRNAs (miRNAs) are crucial regulators of various biological processes and molecular functions. Aberrant miRNA expression has been linked in many studies to neoplastic transformation. Among these miRNAs, dysregulation of miR-301b-5p was associated with different types of cancer including breast cancer. Although many research works have investigated the function of miR-301b in carcinogenesis, few have examined its expression, biological, and clinical implications in breast cancer.
we examined the expression levels of miR-301b-5p in human cancerous breast tissue compared to normal breast controls using different bioinformatic tools and RT-qPCR analyses.
we detected that miR-301b-5p was differentially expressed in cancerous breast tissue when compared to normal controls. MiR-301b-5p was detected to be upregulated in high-grade (Grade 3) and triple-negative breast cancers. A significant strong positive correlation was detected between miR-301b and Ki-67, the commonly used proliferative marker in breast cancer. Bioinformatics analyses using the KM plotter revealed that miR-301b has significant prognostic power in assessing the OS of patients with breast cancer. The study also identified many fundamental biological processes and regulatory pathways associated with the investigated miR-301b-related hub genes. Interestingly, the expression pattern and prognostic significance of PTEN, the top hub gene regulated by miR-301b, highlighted the prognostic significance of PTEN in breast cancer.
The current study findings suggest the potential use of miR-301b-5p as a possible diagnostic and prognostic biomarker in breast cancer. Moreover, this study emphasized the clinical and biological relevance of miR-301b-5p in breast cancer.
微小 RNA（miRNAs）是各种生物过程和分子功能的关键调节因子。许多研究表明，miRNA 的异常表达与肿瘤转化有关。在这些 miRNA 中，miR-301b-5p 的失调与包括乳腺癌在内的多种癌症有关。尽管许多研究工作已经研究了 miR-301b 在致癌作用中的功能，但很少有研究检查其在乳腺癌中的表达、生物学和临床意义。
我们使用不同的生物信息学工具和 RT-qPCR 分析，检测了人癌性乳腺组织与正常乳腺对照中 miR-301b-5p 的表达水平。
我们发现，与正常对照组相比，miR-301b-5p 在癌性乳腺组织中表达差异。miR-301b-5p 在高级别（3 级）和三阴性乳腺癌中被检测到上调。miR-301b 与 Ki-67 之间检测到显著的强正相关，Ki-67 是乳腺癌中常用的增殖标志物。使用 KM 绘图器进行的生物信息学分析表明，miR-301b 在评估乳腺癌患者的 OS 方面具有显著的预后能力。该研究还确定了许多与研究的 miR-301b 相关枢纽基因相关的基本生物学过程和调节途径。有趣的是，PTEN，受 miR-301b 调节的顶级枢纽基因的表达模式和预后意义，突出了 PTEN 在乳腺癌中的预后意义。
本研究结果表明，miR-301b-5p 可能作为乳腺癌的一种潜在诊断和预后生物标志物。此外，本研究强调了 miR-301b-5p 在乳腺癌中的临床和生物学相关性。
