黑腹果蝇中的超氧化物歧化酶。两种电泳变体之间的突变位点差异。

Superoxide dismutase in Drosophila melanogaster. Mutation site difference between two electromorphs.

作者信息

Lee Y M, Ayala F J

出版信息

FEBS Lett. 1985 Jan 1;179(1):115-9. doi: 10.1016/0014-5793(85)80203-9.

Abstract

Two electrophoretically distinguishable variants of superoxide dismutase (SOD) are common in natural populations of Drosophila melanogaster. We have earlier comparatively characterized these two electromorphs, SODF and SODS. By peptide mapping in high-performance liquid chromatography (HPLC), we now show that the difference between the two electromorphs is due to the replacement of Asn-96 (SODF) by Lys-96 (SODS). It is far from clear how this replacement causes the biochemical differences (in thermostability, specific activity, and others) observed between these two forms of the enzyme.

摘要

超氧化物歧化酶（SOD）的两种可通过电泳区分的变体在黑腹果蝇的自然种群中很常见。我们之前已经对这两种电泳形态SODF和SODS进行了比较表征。通过高效液相色谱（HPLC）中的肽图谱分析，我们现在表明这两种电泳形态之间的差异是由于96位的天冬酰胺（SODF）被96位的赖氨酸（SODS）取代。目前尚不清楚这种取代是如何导致在这两种酶形式之间观察到的生化差异（热稳定性、比活性等）的。