College of Engineering, Northeast Agriculture University, Harbin 150030, PR China; School of Environment, Harbin Institute of Technology, Harbin 150001, PR China; Key Laboratory of Swine Facilities Engineering, Ministry of Agriculture and Rural Affairs, Harbin 150030, PR China.
College of Engineering, Northeast Agriculture University, Harbin 150030, PR China; Key Laboratory of Swine Facilities Engineering, Ministry of Agriculture and Rural Affairs, Harbin 150030, PR China.
J Biosci Bioeng. 2024 Nov;138(5):399-405. doi: 10.1016/j.jbiosc.2024.05.012. Epub 2024 Aug 22.
In this study, the optimal hydrogen (H) production conditions of the high-efficiency H-producing mutant strain Ethanoligenens harbinense YR-3 (carbon-nitrogen ratio 5.5, phosphate buffer 80 mM, initial pH 6.0, biotin 1.4 mg/L) are obtained by intermittent experiments. The maximum specific H production rate of YR-3 (2.85 mol H/mol glucose) was 1.4 times that of the wild strain ZGX4 (2.04 mol H/mol glucose). The liquid-phase products are mainly ethanol and acetic acid, indicating that the metabolic pathway has not changed. Two-dimensional electrophoresis and mass spectrometry were used to compare and analyze the protein map differences between YR-3 and ZGX4. The results show that 1,6-fructose diphosphate aldolase and the flavoprotein in hydrogenase are highly expressed. This study will provide a theoretical basis for the genetic modification of high-efficiency H-producing strains and the improvement of H production capacity.
在这项研究中，通过间歇实验获得了高效产氢突变株乙醇发酵杆菌（碳氮比 5.5、磷酸缓冲液 80 mM、初始 pH 值 6.0、生物素 1.4 mg/L）的最佳产氢条件。YR-3 的最大比产氢速率（2.85 mol H/mol 葡萄糖）是野生型菌株 ZGX4（2.04 mol H/mol 葡萄糖）的 1.4 倍。液相产物主要为乙醇和乙酸，表明代谢途径没有改变。二维电泳和质谱用于比较和分析 YR-3 和 ZGX4 之间的蛋白质图谱差异。结果表明，1,6-果糖二磷酸醛缩酶和氢化酶中的黄素蛋白高度表达。本研究将为高效产氢菌株的遗传改造和产氢能力的提高提供理论基础。
