Zhang Jing, Yuan Yue, Wang Zhiwen, Chen Tao
Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
College of Agricultural and Biological Engineering, Heze University, Heze 274000, Shandong, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Aug 25;40(8):2666-2677. doi: 10.13345/j.cjb.240071.
Itaconic acid (IA) is one of the twelve high value-added platform compounds applied in various fields including coatings, adhesives, plastics, resins, and biofuels. In this study, we established a one-pot catalytic synthesis system for IA from citric acid based on the engineered salt-tolerant bacterial strain TDZI-08 after investigating factors that hindered the process and optimizing the carbon source, nitrogen source, inducer addition time, and surfactant dosage. The open, non-sterile, one-pot synthesis with TDZI-08 in a 5 L fermenter achieved the highest IA titer of 40.50 g/L, with a catalytic yield of 0.68 g IA/g citric acid during the catalytic stage and a total yield of 0.42 g IA/g (citric acid+gluconic acid). The one-pot synthesis system established in this study is simple and does not need sterilization or aseptic operations. The findings indicate the potential of . for industrial production of IA.
衣康酸（IA）是十二种高附加值平台化合物之一，应用于涂料、粘合剂、塑料、树脂和生物燃料等各个领域。在本研究中，我们在研究了阻碍该过程的因素并优化了碳源、氮源、诱导剂添加时间和表面活性剂用量后，基于工程化耐盐细菌菌株TDZI - 08建立了一种从柠檬酸一锅法催化合成衣康酸的系统。在5 L发酵罐中使用TDZI - 08进行开放、非无菌的一锅法合成，实现了最高衣康酸滴度为40.50 g/L，催化阶段的催化产率为0.68 g衣康酸/ g柠檬酸，总产率为0.42 g衣康酸/ g（柠檬酸 + 葡萄糖酸）。本研究建立的一锅法合成系统简单，无需灭菌或无菌操作。研究结果表明了……用于衣康酸工业生产的潜力。
