MicroHealth Group, Instituto de Productos Lácteos de Asturias-Consejo Superior de Investigaciones Científicas (IPLA-CSIC)/Instituto Biosanitario del Principado de Asturias (ISPA), Villaviciosa, Asturias, Spain.
Primary Care Center Teatinos-Corredoria, Oviedo, Asturias, Spain.
J Pediatr Gastroenterol Nutr. 2024 Oct;79(4):841-849. doi: 10.1002/jpn3.12356. Epub 2024 Aug 22.
Food protein-induced enterocolitis syndrome (FPIES) is a severe type of non-IgE (immunoglobulin E)-mediated (NIM) food allergy, with cow's milk (CM) being the most common offending food. The relationship between the gut microbiota and its metabolites with the inflammatory process in infants with CM FPIES is unknown, although evidence suggests a microbial dysbiosis in NIM patients. This study was performed to contribute to the knowledge of the interaction between the gut microbiota and its derived metabolites with the local immune system in feces of infants with CM FPIES at diagnosis.
Twelve infants with CM FPIES and a matched healthy control group were recruited and the gut microbiota was investigated by 16S amplicon and shotgun sequencing. Fatty acids (FAs) were measured by gas chromatography, while immune factors were determined by enzyme-linked immunosorbent assay and Luminex technology.
A specific pattern of microbiota in the gut of CM FPIES patients was found, characterized by a high abundance of enterobacteria. Also, an intense excretion of FAs in the feces of these infants was observed. Furthermore, correlations were found between fecal bifidobacteria and immune factors.
These fecal determinations may be useful to gain insight into the pathophysiology of this syndrome and should be taken in consideration for future studies of FPIES patients.
食物蛋白诱导的肠炎综合征（FPIES）是一种严重的非 IgE（免疫球蛋白 E）介导的（NIM）食物过敏，以牛奶（CM）为最常见的致敏食物。虽然有证据表明非 IgE 患者存在微生物失调，但 CM-FPIES 婴儿肠道微生物群及其代谢物与炎症过程之间的关系尚不清楚。本研究旨在探讨 CM-FPIES 婴儿粪便中肠道微生物群及其衍生代谢物与局部免疫系统之间的相互作用。
招募了 12 名 CM-FPIES 婴儿和一组匹配的健康对照组，并通过 16S 扩增子和 shotgun 测序研究了肠道微生物群。通过气相色谱法测量脂肪酸（FA），通过酶联免疫吸附试验和 Luminex 技术测定免疫因子。
发现 CM-FPIES 患者肠道微生物群存在特定模式，以肠杆菌属丰度高为特征。此外，这些婴儿粪便中 FA 的排泄量也很高。此外，粪便双歧杆菌与免疫因子之间存在相关性。
这些粪便检测可能有助于深入了解该综合征的病理生理学，应在未来的 FPIES 患者研究中考虑。
