大鼠抗生长激素释放因子抗血清对甲硫氨酸脑啡肽类似物FK33 - 824诱导的大鼠生长激素分泌的抑制作用。

Inhibition by antiserum to rat growth hormone-releasing factor of growth hormone secretion induced by a met5-enkephalin analog, FK33-824, in rats.

作者信息

Murakami Y, Kato Y, Kabayama Y, Inoue T, Tojo K, Ohta H, Imura H

出版信息

Proc Soc Exp Biol Med. 1985 Jan;178(1):151-4. doi: 10.3181/00379727-178-rc11.

Abstract

A Met5-enkephalin analog, FK33-824 (5, 10 and 20 micrograms/100 g body wt, iv) caused a dose-related increase in plasma growth hormone (GH) in urethane-anesthetized male rats. Pretreatment with cysteamine (30 mg/100 g body wt, sc), a depletor of hypothalamic somatostatin, increased the plasma GH response to FK33-824 (10 micrograms/100 g body wt, iv). Antiserum specific for rat GH-releasing factor (GRF) (0.5 ml/rat, iv) blunted GH release induced by FK33-824 (10 micrograms/100 g body wt, iv) in rats with or without cysteamine pretreatment. These results suggest that GH secretion induced by the opioid peptide is mediated, at least in part, by hypothalamic GRF in the rat.

摘要

一种甲硫氨酸脑啡肽类似物FK33 - 824（静脉注射，剂量为5、10和20微克/100克体重）可使氨基甲酸乙酯麻醉的雄性大鼠血浆生长激素（GH）水平呈剂量依赖性升高。用半胱胺（皮下注射，剂量为30毫克/100克体重）进行预处理，半胱胺是一种下丘脑生长抑素耗竭剂，可增强血浆GH对FK33 - 824（静脉注射，剂量为10微克/100克体重）的反应。对大鼠生长激素释放因子（GRF）具有特异性的抗血清（静脉注射，剂量为0.5毫升/只）可减弱FK33 - 824（静脉注射，剂量为10微克/100克体重）在有或没有半胱胺预处理的大鼠中诱导的GH释放。这些结果表明，阿片肽诱导的GH分泌至少部分是由大鼠下丘脑GRF介导的。