Liang Xuejuan, Tian Qixue, Chen Linglong, Zhang Yanbing, Peng Yanmei
Hunan Academy of Chinese Medicine, Changsha, China.
Hunan Provincial Hospital of Integrated Traditional Chinese and Western Medicine, Changsha, China.
Front Microbiol. 2024 Aug 8;15:1439204. doi: 10.3389/fmicb.2024.1439204. eCollection 2024.
This study aimed to investigate the effects of licorice processing of different Evodiae Fructus (EF) specifications on liver inflammation and oxidative stress associated with the intestinal mucosal microbiota.
The 25 Kunming mice were divided into control (MCN), raw small-flowered Evodiae Fructus (MRSEF), raw medium-flowered EF (MRMEF), licorice-processed small-flowered EF (MLSEF), and licorice-processed medium-flowered EF (MLSEF) groups. The EF intervention groups were given different specifications of EF extract solutions by gavage. After 21 days, indices of liver inflammation and oxidative stress and intestinal mucosal microbiota were measured in mice.
Compared with the MCN, malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) levels were significantly increased in the MRMEF. Although the trends of oxidative stress and inflammatory indexes in the MLSEF and MLMEF were consistent with those in the raw EF groups, the changes were smaller than those in the raw EF groups. Compared to the raw EF groups, the MLSEF and MLMEF showed closer approximations of metabolic function to the MCN. The abundance of in MRMEF was significantly lower than that in the MCN, and it was not significantly different from the MCN after licorice processing. The probiotic was enriched in the MLSEF. The probiotic was enriched in the MLMEF. Correlation analysis revealed significant negative correlations between IL-1β, some metabolic functions and Corynebacterium.
The effects of medium-flowered EF on oxidative stress and inflammatory factors in the liver of mice were stronger than those of small-flowered EF. The licorice processing can reduce this difference by modulating the abundance of and intestinal mucosal metabolic function.
本研究旨在探讨不同规格吴茱萸经甘草炮制后对与肠道黏膜微生物群相关的肝脏炎症和氧化应激的影响。
将25只昆明小鼠分为对照组（MCN）、生小花吴茱萸组（MRSEF）、生中花吴茱萸组（MRMEF）、甘草制小花吴茱萸组（MLSEF）和甘草制中花吴茱萸组（MLSEF）。吴茱萸干预组通过灌胃给予不同规格的吴茱萸提取液。21天后，检测小鼠肝脏炎症、氧化应激指标及肠道黏膜微生物群。
与MCN组相比，MRMEF组丙二醛（MDA）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）水平显著升高。虽然MLSEF组和MLMEF组氧化应激和炎症指标变化趋势与生吴茱萸组一致，但变化幅度小于生吴茱萸组。与生吴茱萸组相比，MLSEF组和MLMEF组代谢功能与MCN组更接近。MRMEF组中某菌丰度显著低于MCN组，经甘草炮制后与MCN组无显著差异。益生菌在MLSEF组富集。益生菌在MLMEF组富集。相关性分析显示IL-1β、部分代谢功能与棒状杆菌之间存在显著负相关。
中花吴茱萸对小鼠肝脏氧化应激和炎症因子的影响强于小花吴茱萸。甘草炮制可通过调节某菌丰度和肠道黏膜代谢功能来缩小这种差异。
