Abdallah Mutaz Mohammed, Yahia Mawada, Tagelsir Ahmed Yousra, Alfaki Mohamed
Microbiology, College of Life Sciences, Northeast Forestry University, Harbin, CHN.
Medical Laboratory Science/Medical Microbiology, University of Khartoum, Khartoum, SDN.
Cureus. 2024 Jul 23;16(7):e65190. doi: 10.7759/cureus.65190. eCollection 2024 Jul.
The wingless-related integration site family (WNT) signaling pathway is critical for tumor progression and development. It is associated with various neoplasms produced by WNT pathway deregulation; WNT5A, a member of the WNT family, has been linked to carcinogenesis, exhibiting either oncogenic or tumor-suppressive effects. The study investigates how the gene affects certain types of cancer. The study aimed to evaluate the potential prognostic significance of WNT5A genes as diagnostic biomarkers for various types of cancer.
We investigated WNT5A gene expression in a pan-cancer analysis using various bioinformatics databases, including GEPIA (Gene Expression Profiling Interactive Analysis), TIMER2 (Tumor Immune Estimation Resource, Version 2), the University of Alabama at Birmingham Cancer Data Analysis Portal UACLAN databases, the Kaplan-Meier (K-M) plotter, cBioPortal, and Gene Set Cancer Analysis (GSCA). We aimed to gain insight into the expression of WNT5A in various tumors and its relationship with immune infiltration, overall survival, and genetic changes. Public datasets validated WNT5A expression in lung squamous cell carcinoma (LUSC) and stomach adenocarcinoma (STAD) samples.
WNT5A pan-cancer analysis was highly expressed in two cancer types, including STAD and LUSC. Additionally, TIMER results showed a positive association of WNT5A with immune cell infiltration in LUSC and STAD. Survival analysis indicated that LUSC cancer exhibits better overall survival, while STAD has lower overall survival levels, which means a poor prognosis in the STAD cancer type. Furthermore, mutation analysis revealed that the WNT5A gene was mutated in 1.4% of cases, with most alterations being deletions followed by amplifications.
The WNT5A gene's high expression in many malignancies, including LUSC and STAD, suggests it could be used as a diagnostic biomarker. This study shows a relationship between WNT5A expression and immune cell abundance in LUSC and STAD. Our pan-cancer analysis of this gene is the first of its type, and it will inform future research, comprehensive investigation, and wet lab experiments.
无翅相关整合位点家族（WNT）信号通路对肿瘤的进展和发展至关重要。它与WNT通路失调产生的各种肿瘤相关；WNT家族成员WNT5A与致癌作用有关，表现出致癌或肿瘤抑制作用。本研究调查该基因如何影响某些类型的癌症。该研究旨在评估WNT5A基因作为各种癌症诊断生物标志物的潜在预后意义。
我们使用各种生物信息学数据库，包括GEPIA（基因表达谱交互式分析）、TIMER2（肿瘤免疫评估资源，版本2）、阿拉巴马大学伯明翰分校癌症数据分析门户UACLAN数据库、Kaplan-Meier（K-M）绘图仪、cBioPortal和基因集癌症分析（GSCA），在泛癌分析中研究WNT5A基因表达。我们旨在深入了解WNT5A在各种肿瘤中的表达及其与免疫浸润、总生存期和基因变化的关系。公共数据集验证了WNT5A在肺鳞状细胞癌（LUSC）和胃腺癌（STAD）样本中的表达。
WNT5A泛癌分析显示在两种癌症类型中高表达，包括STAD和LUSC。此外，TIMER结果显示WNT5A与LUSC和STAD中的免疫细胞浸润呈正相关。生存分析表明，LUSC癌症的总生存期较好，而STAD的总生存期较低，这意味着STAD癌症类型的预后较差。此外，突变分析显示，WNT5A基因在1.4%的病例中发生突变，大多数改变是缺失，其次是扩增。
WNT5A基因在包括LUSC和STAD在内的许多恶性肿瘤中高表达，表明它可作为诊断生物标志物。本研究显示了WNT5A表达与LUSC和STAD中免疫细胞丰度之间的关系。我们对该基因的泛癌分析尚属首次，它将为未来的研究、全面调查和湿实验室实验提供信息。
