School of Science, China University of Geosciences (Beijing), Beijing 100083, China.
School of Pharmaceutical and Chemical Engineering, Taizhou University, Taizhou 318000, China.
Anal Chem. 2024 Sep 3;96(35):14283-14290. doi: 10.1021/acs.analchem.4c03220. Epub 2024 Aug 23.
Innovative signal amplification and transduction play pivotal roles in bioanalysis. Herein, cascading CRISPR/Cas and the nanozyme are integrated with electronic amplification in an organic photoelectrochemical transistor (OPECT) to enable triple signal amplification, which is exemplified by the miRNA-triggered CRISPR/Cas13a system and polyoxometalate nanozyme for OPECT detection of miRNA-21. The CRISPR/Cas13a-enabled release of glucose oxidase could synergize with peroxidase-like SiW to induce catalytic precipitation on the photogate, inhibiting the interfacial mass transfer and thus the significant suppression of the channel current. The as-developed OPECT sensor demonstrates good sensitivity and selectivity for miRNA-21 detection, with a linear range from 1 fM to 10 nM and an ultralow detection limit of 0.53 fM. This study features the integration of bio- and nanoenzyme cascade and electronic triple signal amplification for OPECT detection.
创新的信号放大和转导在生物分析中起着关键作用。在此，级联的 CRISPR/Cas 和纳米酶与电子放大集成在有机光电晶体管（OPECT）中，实现三重信号放大，以 miRNA 触发的 CRISPR/Cas13a 系统和多金属氧酸盐纳米酶为例，用于 OPECT 检测 miRNA-21。CRISPR/Cas13a 使葡萄糖氧化酶释放，与过氧化物酶样 SiW 协同作用，在光栅上诱导催化沉淀，抑制界面传质，从而显著抑制沟道电流。所开发的 OPECT 传感器对 miRNA-21 的检测具有良好的灵敏度和选择性，线性范围从 1 fM 到 10 nM，检测限低至 0.53 fM。本研究的特点是将生物酶和纳米酶级联与电子三重信号放大集成用于 OPECT 检测。
