Cai Ting, Zhang Wenran, Lian Lizhen, Sun Yali, Xia Zihao, Chen Yuxuan, Shuai Jing, Lin Peng, Zhang Qian, Liu Shenghua
School of Materials, Shenzhen Campus of Sun Yat-sen University, Shenzhen, Guangdong 518107, P. R. China.
Songshan Lake Materials Laboratory, Songshan Lake Mat Lab, Dongguan 523808, China.
Anal Chem. 2025 Jan 14;97(1):526-534. doi: 10.1021/acs.analchem.4c04755. Epub 2024 Dec 26.
The integration of a photosensitive gate into an organic electrochemical transistor has currently emerged as a promising route for biological sensing. However, the modification of the photosensitive gate always involves complex processes, and the degradation of sensitivity of the functional materials under illumination will significantly decrease the stability of the devices. Herein, we designed an organic photoelectrochemical transistor (OPECT) biosensor employing horseradish peroxidase (HRP)@glucose oxidase (GOx)/Pt/n-Si as the photosensitive gate based on the "shadow effect". The glucose-dependent hydrogen peroxide with HRP/GOx was modified on the gate electrode, triggering a biocatalytic precipitation reaction, which induces the illumination contrast, resulting in a biologically gating effect on the corresponding channel current response. Thus, high sensitivity and selectivity in glucose detection of the OPECT devices will be realized. Given the easy fabrication and high stability of the Pt/n-Si electrode, it has great potential to become a superior selectivity as an OPECT gate electrode. This work provides conceptual validation for the study of the interaction between the photosensitive gate based on the "shadow effect" and biomolecular sensing, which can further expand the application of the OPECT biosensors under interior lighting and shadow surroundings.
目前，将光敏栅极集成到有机电化学晶体管中已成为生物传感的一条有前景的途径。然而，光敏栅极的修饰总是涉及复杂的过程，并且功能材料在光照下的灵敏度下降会显著降低器件的稳定性。在此，我们基于“阴影效应”设计了一种以辣根过氧化物酶（HRP）@葡萄糖氧化酶（GOx）/Pt/n-Si作为光敏栅极的有机光电化学晶体管（OPECT）生物传感器。将与HRP/GOx相关的葡萄糖依赖性过氧化氢修饰在栅电极上，引发生物催化沉淀反应，从而引起光照对比度，进而对相应的沟道电流响应产生生物门控效应。因此，将实现OPECT器件在葡萄糖检测中的高灵敏度和高选择性。鉴于Pt/n-Si电极易于制备且稳定性高，它作为OPECT栅电极具有成为优异选择性电极的巨大潜力。这项工作为基于“阴影效应”的光敏栅极与生物分子传感之间相互作用的研究提供了概念验证，这可以进一步扩展OPECT生物传感器在室内光照和阴影环境下的应用。
