Department of Dermatology, Shenzhen Qianhai Shekou Free Trade Zone Hospital, Shenzhen, China.
Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Skin Res Technol. 2024 Aug;30(8):e70001. doi: 10.1111/srt.70001.
The specific role of oxidative stress (OS) in vitiligo and alopecia areata (AA) remains unclear. The aim of this study was to analyze and identify the key markers of OS in vitiligo and AA by bioinformatics.
We obtained vitiligo and AA datasets from gene expression omnibus (GEO) database. The difference-expressed genes of vitiligo and AA were identified by differential analysis, and the functions of difference-expressed genes were identified by gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) enrichment analysis. Subsequently, Veen package was used to obtain the intersection genes of OS-related genes with vitiligo and AA. Finally, we used CIBERSORT to assess the infiltration of immune cells in vitiligo and AA.
Through enrichment analysis, we found that vitiligo and AA were mainly enriched in cell cycle and cell adhesion molecular channels. We identified KLB and EIF3C as key genes in OS regulation of vitiligo and AA, and found that KLB and EIF3C participate in disease progression by regulating T cells and neutrophils.
According to our findings, KLB and EIF3C play a crucial role in the progression and development of vitiligo and AA, which have been identified as biomarkers and target for early diagnosis of patients.
氧化应激（OS）在白癜风和斑秃中的具体作用仍不清楚。本研究旨在通过生物信息学方法分析和确定 OS 在白癜风和斑秃中的关键标志物。
我们从基因表达综合数据库（GEO）中获得了白癜风和斑秃的数据集。通过差异分析鉴定白癜风和斑秃的差异表达基因，并通过基因本体论（GO）、京都基因与基因组百科全书（KEGG）和基因集富集分析（GSEA）富集分析鉴定差异表达基因的功能。随后，使用 Veen 包获得与白癜风和斑秃相关的 OS 相关基因的交集基因。最后，我们使用 CIBERSORT 评估白癜风和斑秃中免疫细胞的浸润情况。
通过富集分析，我们发现白癜风和斑秃主要富集在细胞周期和细胞黏附分子通道中。我们鉴定出 KLB 和 EIF3C 是 OS 调节白癜风和斑秃的关键基因，并发现 KLB 和 EIF3C 通过调节 T 细胞和中性粒细胞参与疾病的进展。
根据我们的发现，KLB 和 EIF3C 在白癜风和斑秃的进展和发展中起着关键作用，它们已被确定为患者早期诊断的生物标志物和靶标。
