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从储存的屠宰场废物中提取绵羊角膜支架的可持续方法。

A sustainable approach to derive sheep corneal scaffolds from stored slaughterhouse waste.

机构信息

Department of Biomedical Engineering & Biotechnology, College of Medicine & Health Sciences, Khalifa University of Science & Technology, Abu Dhabi, UAE.

Biomedical Engineering & Healthcare Engineering Innovation Center, Khalifa University, Abu Dhabi, UAE.

出版信息

Regen Med. 2024 Jun 2;19(6):303-315. doi: 10.1080/17460751.2024.2357499. Epub 2024 Jun 10.

DOI:10.1080/17460751.2024.2357499
PMID:39177571
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11346552/
Abstract

The escalating demand for corneal transplants significantly surpasses the available supply. To bridge this gap, we concentrated on ethical and sustainable corneal grafting sources. Our objective was to create viable corneal scaffolds from preserved slaughterhouse waste. Corneas were extracted and decellularized from eyeballs that had been refrigerated for several days. These scaffolds underwent evaluation through DNA quantification, histological analysis, surface tension measurement, light propagation testing, and tensile strength assessment. Both the native and acellular corneas (with ~90% DNA removed using a cost-effective and environmentally friendly surfactant) maintained essential optical and biomechanical properties for potential clinical use. Our method of repurposing slaughterhouse waste, stored at 4°C for several days, to develop corneal scaffolds offers a sustainable and economical alternative xenograft model.

摘要

对于角膜移植的需求不断增加，而可供移植的角膜严重短缺。为了解决这一问题，我们专注于寻找伦理且可持续的角膜供体来源。我们的目标是利用屠宰场废弃物来制备可用于移植的角膜支架。我们从冷藏保存数天的眼球中提取并脱细胞化角膜，然后对这些支架进行 DNA 定量、组织学分析、表面张力测量、光传播测试和拉伸强度评估。天然和脱细胞角膜（使用经济环保的表面活性剂去除约 90%的 DNA）均保留了潜在临床应用所必需的光学和生物力学性能。我们的方法是将冷藏保存数天的屠宰场废弃物重新利用来制备角膜支架，这为开发可持续且经济的异种移植物模型提供了一种选择。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/de66758c1f84/IRME_A_2357499_F0008_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/7cfb83577e14/IRME_A_2357499_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/312c91b65a1f/IRME_A_2357499_F0002_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/00e0b2b4a6c6/IRME_A_2357499_F0003_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/dea3686b8627/IRME_A_2357499_F0004_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/5d309b98c0b1/IRME_A_2357499_F0005_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/17b581a41a01/IRME_A_2357499_F0006_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/bcf8961e80d1/IRME_A_2357499_F0007_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/de66758c1f84/IRME_A_2357499_F0008_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/7cfb83577e14/IRME_A_2357499_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/312c91b65a1f/IRME_A_2357499_F0002_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/00e0b2b4a6c6/IRME_A_2357499_F0003_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/dea3686b8627/IRME_A_2357499_F0004_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/5d309b98c0b1/IRME_A_2357499_F0005_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/17b581a41a01/IRME_A_2357499_F0006_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/bcf8961e80d1/IRME_A_2357499_F0007_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e5c/11346552/de66758c1f84/IRME_A_2357499_F0008_C.jpg

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2
A proposed model of xeno-keratoplasty using 3D printing and decellularization.一种使用3D打印和去细胞化技术的异种角膜移植术的建议模型。
Front Pharmacol. 2023 Sep 20;14:1193606. doi: 10.3389/fphar.2023.1193606. eCollection 2023.
3
Gray-Level Co-occurrence Matrix Analysis of Nuclear Textural Patterns in Laryngeal Squamous Cell Carcinoma: Focus on Artificial Intelligence Methods.
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Sci Rep. 2024 Oct 27;14(1):25600. doi: 10.1038/s41598-024-77783-y.
基于灰度共生矩阵分析喉鳞状细胞癌核纹理特征：聚焦人工智能方法。
Microsc Microanal. 2023 Jun 9;29(3):1220-1227. doi: 10.1093/micmic/ozad042.
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3D bioprinting of corneal decellularized extracellular matrix: GelMA composite hydrogel for corneal stroma engineering.角膜脱细胞细胞外基质的3D生物打印：用于角膜基质工程的GelMA复合水凝胶
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