Lauren 分类中用于预测胃癌预后的差异表达 microRNA

Differentially expressed microRNA in prognosis of gastric cancer with Lauren classification.

机构信息

Department of Radiation Oncology, First Affiliated Hospital of Soochow University, Suzhou, China.

Department of Oncology, Affiliated Yixing Hospital of Jiangsu University, Wuxi, China.

出版信息

Cancer Biomark. 2024;41(1):41-54. doi: 10.3233/CBM-230303.

DOI:10.3233/CBM-230303

PMID:39177588

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11492042/

Abstract

BACKGROUND

Gastric cancer (GC) is one of the most common tumors. There were several classifications of GC recently. The value of Lauren classification in evaluating the prognosis after radical gastrectomy was still unclear and the prognosis of gastric cancer remained relatively poor in the absence of prognostic biomarkers. This study aimed to explore microRNA (miRNA) in the prognosis of GC with different Lauren classification.

METHODS

A retrospective study of 1144 patients was performed in this study. Quantificational reverse transcription-PCR (qRT-PCR) was used to examine the expression of miRNAs. Univariate and multivariate analysis were performed to evaluate prognosis value of Lauren classification.

RESULTS

Total 1144 GC patients were recruited in this cohort, including 302 diffuse type (26.4%), 436 intestinal type (38.1%) and 406 mixed type (35.5%) GC. Multivariate analysis showed that Lauren classification, patients' age, tumor size, tumor infiltrating depth, vascular nerve infiltrating and metastatic lymph nodes ration were significantly correlated with GC patients' OS and DFS. The miR-141-3p, miR-200b-3p and miR-133a-5p were significantly down-regulated in diffuse type compared to intestinal type GC tissues, the miR-105-5p had significant lower expression in diffuse type compared with intestinal type and mixed type GC tissues. As a consequence of univariate analysis, low miR-141-3p in diffuse type GC showed significant worse OS and DFS than high miR-141-3p.

CONCLUSIONS

Lauren classification was an independent prognostic factor in GC. MiR-141-3p was an independent prognostic factor and a promising prognostic biomarker in Lauren classification GC.

摘要

背景

胃癌（GC）是最常见的肿瘤之一。最近有几种 GC 分类。Lauren 分类在评估根治性胃切除术后预后中的价值尚不清楚，而且在没有预后生物标志物的情况下，胃癌的预后仍然相对较差。本研究旨在探讨不同 Lauren 分类 GC 中 microRNA（miRNA）的预后价值。

方法

本研究进行了一项回顾性研究，共纳入 1144 例患者。采用定量逆转录聚合酶链反应（qRT-PCR）检测 miRNA 的表达。采用单因素和多因素分析评估 Lauren 分类的预后价值。

结果

本队列共纳入 1144 例 GC 患者，包括弥漫型 302 例（26.4%）、肠型 436 例（38.1%）和混合型 406 例（35.5%）。多因素分析显示，Lauren 分类、患者年龄、肿瘤大小、肿瘤浸润深度、血管神经浸润和转移性淋巴结比率与 GC 患者的 OS 和 DFS 显著相关。与肠型 GC 组织相比，弥漫型 GC 组织中 miR-141-3p、miR-200b-3p 和 miR-133a-5p 显著下调，miR-105-5p 在弥漫型 GC 中与肠型和混合型 GC 组织相比表达显著降低。单因素分析结果显示，弥漫型 GC 中低 miR-141-3p 表达的 OS 和 DFS 明显差于高 miR-141-3p 表达。