Zhang Lan, Huang Zebo, Zhang Huo, Zhu Mingxia, Zhu Wei, Zhou Xin, Liu Ping
Cancer Biomark. 2017;18(3):221-230. doi: 10.3233/CBM-160091.
Studies have reported the prognostic value of dysregulated microRNAs (miRNAs) in gastric cancer (GC). However, the results demonstrated so far are inconsistent.
To better understand the miRNAs with prognostic relevance.
Evaluable miRNAs were selected based on our selection criteria and further analyzed in formalin-fixed paraffin-embedded (FFPE) tissue samples of 169 GC patients using quantitative real-time polymerase chain reaction (qRT-PCR).
A total of 19 miRNAs were selected as candidate miRNAs. Among those miRNAs identified, high expression of miR-21-5p was related to poor overall survival (OS) and disease free survival (DFS) and was identified as an independent prognostic factor. Cases with high level of miR-200c-3p showed poor DFS. Subgroup analysis revealed that high expression of miR-21-5p and miR-222-3p was associated with poor OS and DFS in GC patients not received adjuvant chemotherapy. In male patients, high expression level of miR-21-5p was related to poor OS and DFS.
The present study confirmed that elevated level of miR-21-5p could serve as an independent predictor for poor OS and DFS of GC patients. Moreover, miR-200c-3p, miR-222-3p might also play important roles in the prognosis of GC patients. Further studies are warranted to validate our findings and identify the functions and mechanisms of these miRNAs.
研究报道了失调的微小RNA（miRNA）在胃癌（GC）中的预后价值。然而，目前所展示的结果并不一致。
为了更好地了解具有预后相关性的miRNA。
根据我们的选择标准挑选出可评估的miRNA，并使用定量实时聚合酶链反应（qRT-PCR）在169例GC患者的福尔马林固定石蜡包埋（FFPE）组织样本中进一步分析。
总共19种miRNA被选为候选miRNA。在这些鉴定出的miRNA中，miR-21-5p的高表达与较差的总生存期（OS）和无病生存期（DFS）相关，并被确定为独立的预后因素。miR-200c-3p水平高的病例显示DFS较差。亚组分析显示，在未接受辅助化疗的GC患者中，miR-21-5p和miR-222-3p的高表达与较差的OS和DFS相关。在男性患者中，miR-21-5p的高表达水平与较差的OS和DFS相关。
本研究证实miR-21-5p水平升高可作为GC患者OS和DFS较差的独立预测指标。此外，miR-200c-3p、miR-222-3p可能在GC患者的预后中也发挥重要作用。有必要进一步研究以验证我们的发现，并确定这些miRNA的功能和机制。
