Department of Clinical Oncology, Greater Poland Cancer Centre, 61-866 Poznan, Poland.
Department of Electroradiology, Poznan University of Medical Sciences, 61-701 Poznan, Poland.
Int J Mol Sci. 2024 Jul 24;25(15):8057. doi: 10.3390/ijms25158057.
Gastric cancer (GC) is one of the most frequently diagnosed cancers in the world. Although the incidence is decreasing in developed countries, the treatment results are still unsatisfactory. The standard treatment for locally advanced gastric cancer (LAGC) is gastrectomy with perioperative chemotherapy. The association of selected microRNAs (miRNAs) with chemoresistance was assessed using archival material of patients with LAGC. Histological material was obtained from each patient via a biopsy performed during gastroscopy and then after surgery, which was preceded by four cycles of neoadjuvant chemotherapy (NAC) according to the FLOT or FLO regimen. The expression of selected miRNAs in the tissue material was assessed, including , , , , , , , , , , , and . miRNA expression was assessed using quantitative reverse transcription polymerase chain reaction (qRT-PCR). The response to NAC was assessed using computed tomography of the abdomen and chest and histopathology after gastrectomy. The statistical analyses were performed using GraphPad Prism 9. The significance limit was set at < 0.05. We showed that the expression of , , and before surgery, and and after surgery, decreased in patients with GC. The expression of , , , and decreased in several patients who responded to NAC. The miRNA most commonly expressed in these cases was . Moreover, it showed expression in a patient whose response to chemotherapy was inconsistent between the histopathological results and computed tomography. The expression of , , , and in formalin-fixed paraffin-embedded tissue (FFPET) samples can help differentiate between the responders and non-responders to NAC in LAGC. , , and expression may be used as a potential diagnostic tool in GC patients. The presence of may support the correct assessment of a response to NAC in GC via CT.
胃癌（GC）是世界上最常见的癌症之一。尽管在发达国家发病率正在下降，但治疗效果仍不尽如人意。局部晚期胃癌（LAGC）的标准治疗方法是胃切除术加围手术期化疗。使用 LAGC 患者的存档材料评估选定 microRNAs（miRNAs）与化疗耐药性的相关性。通过内窥镜检查期间进行的活检获得每位患者的组织材料，然后在手术前进行四个周期的新辅助化疗（NAC），根据 FLOT 或 FLO 方案进行。使用定量逆转录聚合酶链反应（qRT-PCR）评估组织材料中选定的 miRNA 表达。使用腹部和胸部计算机断层扫描以及胃切除术后的组织病理学评估 NAC 的反应。使用 GraphPad Prism 9 进行统计分析。显著性限定为 <0.05。我们表明，术前手术前 和 以及术后 和 的表达在 GC 患者中降低。在对 NAC 有反应的几个患者中， 和 、 和 的表达降低。在这些情况下最常表达的 miRNA 是 。此外，它在一个对化疗反应在组织病理学结果和计算机断层扫描之间不一致的患者中表达。在福尔马林固定石蜡包埋组织（FFPET）样本中表达 、 、 和 可以帮助区分 LAGC 中对 NAC 的反应者和非反应者。 和 的表达可能可作为 GC 患者的潜在诊断工具。 的存在可能通过 CT 支持对 GC 中 NAC 反应的正确评估。
