Modern Research Center for Traditional Chinese Medicine, Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, PR China.
Modern Research Center for Traditional Chinese Medicine, Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, PR China; Research Center for Traditional Chinese Medicine Resources and Ethnic Minority Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, PR China.
Bioorg Chem. 2024 Nov;152:107732. doi: 10.1016/j.bioorg.2024.107732. Epub 2024 Aug 16.
Phytochemical analysis of the peeled stems of Syringa pinnatifolia Hemsl. led to the discovery of 13 undescribed lignans, namely helanols A and B (1 and 2) and alashanenols W-G (3-13), as well as four known analogues, of which helanols A and B were lignans with novel skeleton of α-β' linkage. The structures were unambiguously established by extensive spectroscopic analyses, NMR calculations, ECD calculations, and single crystal X-ray crystallography. Five lignans (1, 2, 5, 11 and 13) exhibited a moderate protective effect against HO-induced oxidative injuries in H9c2 cells with the protective rates of 11.3-20.6 % at the concentration of 0.3-20 μM, while the positive control quercetin showed protective rates of 58.7 % at 10 μM. Further mechanism investigation suggested that 1 and 2 exerted the protective effect by regulating the expression of Nrf2/HO-1.
对 Syringa pinnatifolia Hemsl. 的去皮茎进行的植物化学分析导致发现了 13 种未描述的木脂素，分别为 helanols A 和 B（1 和 2）以及 alashanenols W-G（3-13），以及 4 种已知类似物，其中 helanols A 和 B 是具有新颖 α-β' 键连接骨架的木脂素。通过广泛的光谱分析、NMR 计算、ECD 计算和单晶 X 射线晶体学，明确确定了结构。五种木脂素（1、2、5、11 和 13）在 0.3-20 μM 浓度下对 HO 诱导的 H9c2 细胞氧化损伤表现出适度的保护作用，保护率为 11.3-20.6%，而阳性对照槲皮素在 10 μM 时的保护率为 58.7%。进一步的机制研究表明，1 和 2 通过调节 Nrf2/HO-1 的表达发挥保护作用。
