State Key Laboratory of Animal Nutrition and Feeding, SKLANF, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
State Key Laboratory of Animal Nutrition and Feeding, SKLANF, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Ecotoxicol Environ Saf. 2024 Oct 1;284:116901. doi: 10.1016/j.ecoenv.2024.116901. Epub 2024 Aug 22.
Glyphosate is the active ingredient in the herbicide (i.e., Roundup, Touchdown and Erasure), the safety of which has become a social concern. Hawthorn-leaf flavonoid (HF) possesses various biological functions, including antioxidant, regulating lipid metabolism and intestinal microbiota. Whether HF could reduce the health risk of pure glyphosate to birds remain unknown. The experiment aimed to evaluate the effects of pure glyphosate (25 mg/kg added to water) on the intestinal health and microbiota of chicks and the protective roles of HF (60 mg/kg added to the diet). Exposure to glyphosate decreased growth performance, ileal morphology structure, and antioxidant capacity, and increased the serum level of lipid and pro-inflammatory factors. 16S rRNA sequencing indicated that glyphosate decreased bacterial richness and the abundance of Lactobacillus, and increased proportions of pathogens in the ileum. Metabolomic results revealed that glyphosate increased the level of the cholic acid and fatty acids in the ileac digesta. Meanwhile, glyphosate down-regulated the protein expression associated with lipid transport, antioxidant and tight junction in the ileal mucosal tissue, and up-regulated the pro-inflammatory, oxidative stress proteins. However, dietary HF supplementation effectively mitigated the adverse effects of glyphosate and improved intestinal health of chicks. Therefore, dietary HF can ameliorate the harmful effects of glyphosate on birds, which highlights the potential application of HF in reducing the health risks.
草甘膦是除草剂（如 Roundup、Touchdown 和 Erasure）的有效成分，其安全性已成为社会关注的焦点。山楂叶黄酮（HF）具有多种生物学功能，包括抗氧化、调节脂质代谢和肠道微生物群。HF 是否能降低纯草甘膦对鸟类的健康风险尚不清楚。本实验旨在评估纯草甘膦（以 25mg/kg 的量添加到水中）对小鸡肠道健康和微生物群的影响，以及 HF（以 60mg/kg 的量添加到饮食中）的保护作用。暴露于草甘膦会降低生长性能、回肠形态结构和抗氧化能力，并增加血清中脂质和促炎因子的水平。16S rRNA 测序表明，草甘膦降低了回肠中细菌的丰富度和乳酸菌的丰度，增加了病原体的比例。代谢组学结果表明，草甘膦增加了回肠内容物中胆酸和脂肪酸的水平。同时，草甘膦下调了回肠黏膜组织中与脂质转运、抗氧化和紧密连接相关的蛋白表达，并上调了促炎、氧化应激蛋白。然而，饮食中补充 HF 可有效减轻草甘膦的不良影响，改善小鸡的肠道健康。因此，饮食中补充 HF 可以减轻草甘膦对鸟类的有害影响，这凸显了 HF 在降低健康风险方面的潜在应用。
