Bernutz C, Kewenig M, Horn K, Pickardt C R
Clin Chem. 1985 Feb;31(2):289-92.
To measure the concentrations of thyrotropin (thyroid-stimulating hormone), we used the components of a commercially available two-step "sandwich" enzyme immunoassay (Enzymun-Test TSH, Boehringer Mannheim) based on the specific binding of the beta-subunit of thyrotropin by monoclonal antibodies coated on polystyrene tubes. By modifying the original assay protocol, we lowered the limit of detection to 0.18 milli-int. units/L, using a total incubation period of 22 h. With this modification we could differentiate between patients responsive to administration of thyroliberin (thyrotropin-releasing factor) and those who were non-responders, by measuring only the basal concentration of thyrotropin. Furthermore, we demonstrated a correlation between the basal concentration of thyrotropin and its increase after administration of thyroliberin (r = 0.77, n = 48).
为测定促甲状腺素（甲状腺刺激激素）的浓度，我们使用了市售两步“夹心”酶免疫测定法（Enzymun-Test TSH，宝灵曼公司）的组件，该方法基于包被在聚苯乙烯管上的单克隆抗体对促甲状腺素β亚基的特异性结合。通过修改原始检测方案，我们将检测限降低至0.18毫国际单位/升，总孵育时间为22小时。通过这种修改，我们仅通过测量促甲状腺素的基础浓度，就能区分对促甲状腺素释放素（促甲状腺激素释放因子）给药有反应的患者和无反应的患者。此外，我们证明了促甲状腺素基础浓度与其在给予促甲状腺素释放素后的升高之间存在相关性（r = 0.77，n = 48）。
